| Title: |
Assessment of Fcγ receptor-dependent binding of influenza hemagglutinin vaccine-induced antibodies in a non-human primate model |
| Authors: |
Yuji Masuta, Shokichi Takahama, Takuto Nogimori, Saya Moriyama, Yoshimasa Takahashi, Takuya Yamamoto |
| Source: |
iScience, Vol 25, Iss 10, Pp 105085- (2022) |
| Publisher Information: |
Elsevier, 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Science |
| Subject Terms: |
Biological sciences, Immunology, Immunological methods, Science |
| More Details: |
Summary: Several cross-protective antibodies that recognize a broad range of influenza A virus (IAV) strains are known to have functions in virus elimination such as Fcγ receptor (FcγR)-effector function and neutralizing activity against the head region. Although few studies have used primary cells as effector cells, the FcγR-effector function was evaluated after isolating each cell subset. Herein, we established an original assay system to evaluate purified FI6 IgG-mediated binding to hemagglutinin (HA)-expressing cells by flow cytometry using peripheral blood mononuclear cells from cynomolgus macaques. In addition, we evaluated the FcγR-effector function of IAV vaccine-induced anti-HA antibodies in cynomolgus macaques after administering the split vaccine. We found several cell types, mainly classical monocytes, bound to HA-expressing target cells in an FcγR-dependent manner, that were dominant in the binding of the cell population. Thus, this assay system could facilitate the development of a universal influenza vaccine. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2589-0042 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2589004222013578; https://doaj.org/toc/2589-0042 |
| DOI: |
10.1016/j.isci.2022.105085 |
| Access URL: |
https://doaj.org/article/d46b953959d045d888ad922f93e1bb2a |
| Accession Number: |
edsdoj.46b953959d045d888ad922f93e1bb2a |
| Database: |
Directory of Open Access Journals |
