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Proton Detected Solid-State NMR of Membrane Proteins at 28 Tesla (1.2 GHz) and 100 kHz Magic-Angle Spinning

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Title: Proton Detected Solid-State NMR of Membrane Proteins at 28 Tesla (1.2 GHz) and 100 kHz Magic-Angle Spinning
Authors: Evgeny Nimerovsky, Kumar Tekwani Movellan, Xizhou Cecily Zhang, Marcel C. Forster, Eszter Najbauer, Kai Xue, Rıza Dervişoǧlu, Karin Giller, Christian Griesinger, Stefan Becker, Loren B. Andreas
Source: Biomolecules, Vol 11, Iss 5, p 752 (2021)
Publisher Information: MDPI AG, 2021.
Publication Year: 2021
Collection: LCC:Microbiology
Subject Terms: magic-angle spinning, solid-state NMR, membrane protein, beta barrel, transmembrane, proton detection, Microbiology, QR1-502
More Details: The available magnetic field strength for high resolution NMR in persistent superconducting magnets has recently improved from 23.5 to 28 Tesla, increasing the proton resonance frequency from 1 to 1.2 GHz. For magic-angle spinning (MAS) NMR, this is expected to improve resolution, provided the sample preparation results in homogeneous broadening. We compare two-dimensional (2D) proton detected MAS NMR spectra of four membrane proteins at 950 and 1200 MHz. We find a consistent improvement in resolution that scales superlinearly with the increase in magnetic field for three of the four examples. In 3D and 4D spectra, which are now routinely acquired, this improvement indicates the ability to resolve at least 2 and 2.5 times as many signals, respectively.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2218-273X
Relation: https://www.mdpi.com/2218-273X/11/5/752; https://doaj.org/toc/2218-273X
DOI: 10.3390/biom11050752
Access URL: https://doaj.org/article/01ea7bb4bacb41918cf37118af2d9c91
Accession Number: edsdoj.01ea7bb4bacb41918cf37118af2d9c91
Database: Directory of Open Access Journals
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  Data: Biomolecules, Vol 11, Iss 5, p 752 (2021)
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  Data: The available magnetic field strength for high resolution NMR in persistent superconducting magnets has recently improved from 23.5 to 28 Tesla, increasing the proton resonance frequency from 1 to 1.2 GHz. For magic-angle spinning (MAS) NMR, this is expected to improve resolution, provided the sample preparation results in homogeneous broadening. We compare two-dimensional (2D) proton detected MAS NMR spectra of four membrane proteins at 950 and 1200 MHz. We find a consistent improvement in resolution that scales superlinearly with the increase in magnetic field for three of the four examples. In 3D and 4D spectra, which are now routinely acquired, this improvement indicates the ability to resolve at least 2 and 2.5 times as many signals, respectively.
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