Bibliographic Details
| Title: |
Proton Detected Solid-State NMR of Membrane Proteins at 28 Tesla (1.2 GHz) and 100 kHz Magic-Angle Spinning |
| Authors: |
Evgeny Nimerovsky, Kumar Tekwani Movellan, Xizhou Cecily Zhang, Marcel C. Forster, Eszter Najbauer, Kai Xue, Rıza Dervişoǧlu, Karin Giller, Christian Griesinger, Stefan Becker, Loren B. Andreas |
| Source: |
Biomolecules, Vol 11, Iss 5, p 752 (2021) |
| Publisher Information: |
MDPI AG, 2021. |
| Publication Year: |
2021 |
| Collection: |
LCC:Microbiology |
| Subject Terms: |
magic-angle spinning, solid-state NMR, membrane protein, beta barrel, transmembrane, proton detection, Microbiology, QR1-502 |
| More Details: |
The available magnetic field strength for high resolution NMR in persistent superconducting magnets has recently improved from 23.5 to 28 Tesla, increasing the proton resonance frequency from 1 to 1.2 GHz. For magic-angle spinning (MAS) NMR, this is expected to improve resolution, provided the sample preparation results in homogeneous broadening. We compare two-dimensional (2D) proton detected MAS NMR spectra of four membrane proteins at 950 and 1200 MHz. We find a consistent improvement in resolution that scales superlinearly with the increase in magnetic field for three of the four examples. In 3D and 4D spectra, which are now routinely acquired, this improvement indicates the ability to resolve at least 2 and 2.5 times as many signals, respectively. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2218-273X |
| Relation: |
https://www.mdpi.com/2218-273X/11/5/752; https://doaj.org/toc/2218-273X |
| DOI: |
10.3390/biom11050752 |
| Access URL: |
https://doaj.org/article/01ea7bb4bacb41918cf37118af2d9c91 |
| Accession Number: |
edsdoj.01ea7bb4bacb41918cf37118af2d9c91 |
| Database: |
Directory of Open Access Journals |
