| Title: |
One-pot biosynthesis of N-acetylneuraminic acid from chitin via combination of chitin-degrading enzymes, N-acetylglucosamin e-2-epimerase, and N-neuraminic acid aldolase. |
| Authors: |
Quanzhen Liu, Guoguang Wei, Pengfan Yang, Chengyong Wang, Kequan Chen, Pingkai Ouyang, Alei Zhang |
| Source: |
Frontiers in Microbiology; 3/21/2023, Vol. 14, p1-7, 7p |
| Subject Terms: |
MULTIENZYME complexes, BIOSYNTHESIS, CHITIN, DIETARY supplements, ENZYMES, ACIDS |
| Abstract: |
N-acetylneuraminic acid (Neu5Ac) possesses the ability to promote mental health and enhance immunity and is widely used in both medicine and food fields as a supplement. Enzymatic production of Neu5Ac using N-acetyl-D-glucosamine (GlcNAc) as substrate was significant. However, the high-cost GlcNAc limited its development. In this study, an in vitro multi-enzyme catalysis was built to produce Neu5Ac using affordable chitin as substrate. Firstly, exochitinase SmChiA from Serratia proteamaculans and N-acetylglucosaminosidase CmNAGase from Chitinolyticbacter meiyuanensis SYBC-H1 were screened and combined to produce GlcNAc, effectively. Then, the chitinase was cascaded with N-acetylglucosamine-2-epimerase (AGE) and N-neuraminic acid aldolase (NanA) to produce Neu5Ac; the optimal conditions of the multi-enzyme catalysis system were 37°C and pH 8.5, the ratio of AGE to NanA (1:4) and addition of pyruvate (70 mM), respectively. Finally, 9.2 g/L Neu5Ac could be obtained from 20 g/L chitin within 24 h along with two supplementations with pyruvate. This work will lay a good foundation for the production of Neu5Ac from cheap chitin resources. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
