One-pot biosynthesis of N-acetylneuraminic acid from chitin via combination of chitin-degrading enzymes, N-acetylglucosamin e-2-epimerase, and N-neuraminic acid aldolase.
| Title: | One-pot biosynthesis of N-acetylneuraminic acid from chitin via combination of chitin-degrading enzymes, N-acetylglucosamin e-2-epimerase, and N-neuraminic acid aldolase. |
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| Authors: | Quanzhen Liu, Guoguang Wei, Pengfan Yang, Chengyong Wang, Kequan Chen, Pingkai Ouyang, Alei Zhang |
| Source: | Frontiers in Microbiology; 3/21/2023, Vol. 14, p1-7, 7p |
| Subject Terms: | MULTIENZYME complexes, BIOSYNTHESIS, CHITIN, DIETARY supplements, ENZYMES, ACIDS |
| Abstract: | N-acetylneuraminic acid (Neu5Ac) possesses the ability to promote mental health and enhance immunity and is widely used in both medicine and food fields as a supplement. Enzymatic production of Neu5Ac using N-acetyl-D-glucosamine (GlcNAc) as substrate was significant. However, the high-cost GlcNAc limited its development. In this study, an in vitro multi-enzyme catalysis was built to produce Neu5Ac using affordable chitin as substrate. Firstly, exochitinase SmChiA from Serratia proteamaculans and N-acetylglucosaminosidase CmNAGase from Chitinolyticbacter meiyuanensis SYBC-H1 were screened and combined to produce GlcNAc, effectively. Then, the chitinase was cascaded with N-acetylglucosamine-2-epimerase (AGE) and N-neuraminic acid aldolase (NanA) to produce Neu5Ac; the optimal conditions of the multi-enzyme catalysis system were 37°C and pH 8.5, the ratio of AGE to NanA (1:4) and addition of pyruvate (70 mM), respectively. Finally, 9.2 g/L Neu5Ac could be obtained from 20 g/L chitin within 24 h along with two supplementations with pyruvate. This work will lay a good foundation for the production of Neu5Ac from cheap chitin resources. [ABSTRACT FROM AUTHOR] |
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| Database: | Complementary Index |
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| Items | – Name: Title Label: Title Group: Ti Data: One-pot biosynthesis of N-acetylneuraminic acid from chitin via combination of chitin-degrading enzymes, N-acetylglucosamin e-2-epimerase, and N-neuraminic acid aldolase. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Quanzhen+Liu%22">Quanzhen Liu</searchLink><br /><searchLink fieldCode="AR" term="%22Guoguang+Wei%22">Guoguang Wei</searchLink><br /><searchLink fieldCode="AR" term="%22Pengfan+Yang%22">Pengfan Yang</searchLink><br /><searchLink fieldCode="AR" term="%22Chengyong+Wang%22">Chengyong Wang</searchLink><br /><searchLink fieldCode="AR" term="%22Kequan+Chen%22">Kequan Chen</searchLink><br /><searchLink fieldCode="AR" term="%22Pingkai+Ouyang%22">Pingkai Ouyang</searchLink><br /><searchLink fieldCode="AR" term="%22Alei+Zhang%22">Alei Zhang</searchLink> – Name: TitleSource Label: Source Group: Src Data: Frontiers in Microbiology; 3/21/2023, Vol. 14, p1-7, 7p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22MULTIENZYME+complexes%22">MULTIENZYME complexes</searchLink><br /><searchLink fieldCode="DE" term="%22BIOSYNTHESIS%22">BIOSYNTHESIS</searchLink><br /><searchLink fieldCode="DE" term="%22CHITIN%22">CHITIN</searchLink><br /><searchLink fieldCode="DE" term="%22DIETARY+supplements%22">DIETARY supplements</searchLink><br /><searchLink fieldCode="DE" term="%22ENZYMES%22">ENZYMES</searchLink><br /><searchLink fieldCode="DE" term="%22ACIDS%22">ACIDS</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: N-acetylneuraminic acid (Neu5Ac) possesses the ability to promote mental health and enhance immunity and is widely used in both medicine and food fields as a supplement. Enzymatic production of Neu5Ac using N-acetyl-D-glucosamine (GlcNAc) as substrate was significant. However, the high-cost GlcNAc limited its development. In this study, an in vitro multi-enzyme catalysis was built to produce Neu5Ac using affordable chitin as substrate. Firstly, exochitinase SmChiA from Serratia proteamaculans and N-acetylglucosaminosidase CmNAGase from Chitinolyticbacter meiyuanensis SYBC-H1 were screened and combined to produce GlcNAc, effectively. Then, the chitinase was cascaded with N-acetylglucosamine-2-epimerase (AGE) and N-neuraminic acid aldolase (NanA) to produce Neu5Ac; the optimal conditions of the multi-enzyme catalysis system were 37°C and pH 8.5, the ratio of AGE to NanA (1:4) and addition of pyruvate (70 mM), respectively. Finally, 9.2 g/L Neu5Ac could be obtained from 20 g/L chitin within 24 h along with two supplementations with pyruvate. This work will lay a good foundation for the production of Neu5Ac from cheap chitin resources. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Frontiers in Microbiology is the property of Frontiers Media S.A. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3389/fmicb.2023.1156924 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 7 StartPage: 1 Subjects: – SubjectFull: MULTIENZYME complexes Type: general – SubjectFull: BIOSYNTHESIS Type: general – SubjectFull: CHITIN Type: general – SubjectFull: DIETARY supplements Type: general – SubjectFull: ENZYMES Type: general – SubjectFull: ACIDS Type: general Titles: – TitleFull: One-pot biosynthesis of N-acetylneuraminic acid from chitin via combination of chitin-degrading enzymes, N-acetylglucosamin e-2-epimerase, and N-neuraminic acid aldolase. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Quanzhen Liu – PersonEntity: Name: NameFull: Guoguang Wei – PersonEntity: Name: NameFull: Pengfan Yang – PersonEntity: Name: NameFull: Chengyong Wang – PersonEntity: Name: NameFull: Kequan Chen – PersonEntity: Name: NameFull: Pingkai Ouyang – PersonEntity: Name: NameFull: Alei Zhang IsPartOfRelationships: – BibEntity: Dates: – D: 21 M: 03 Text: 3/21/2023 Type: published Y: 2023 Identifiers: – Type: issn-print Value: 1664302X Numbering: – Type: volume Value: 14 Titles: – TitleFull: Frontiers in Microbiology Type: main |
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