| Title: |
DARESOME enables concurrent profiling of multiple DNA modifications with restriction enzymes in single cells and cell-free DNA. |
| Authors: |
Viswanathan, Ramya1,2, Cheruba, Elsie1,2, Pui-Mun Wong3, Yao Yi4, Shaun Ngang1,2, Dawn Qingqing Chong5,6, Yuin-Han Loh4,7, Tan, Iain Beehuat3,5,6, Lih Feng Cheow1,2 lihfeng.cheow@nus.edu.sg |
| Source: |
Science Advances. 9/13/2023, Vol. 9 Issue 37, p1-14. 14p. |
| Abstract: |
The article discusses DNA modifications, specifically 5-methylcytosines (5mCs) and 5-hydroxymethylcytosine (5hmC), which play crucial roles in gene regulation and epigenetics. It highlights the challenges in distinguishing between these modifications in DNA sequencing and explores various methods, including restriction enzyme-based assays, for accurate measurement and profiling of these modifications, allowing for a better understanding of their roles in cellular functions. |
| Database: |
Academic Search Complete |
