Bibliographic Details
| Title: |
Correlation of ROS1 Immunohistochemistry With ROS1 Fusion Status Determined by Fluorescence In Situ Hybridization. |
| Authors: |
Huang, Richard S. P., Smith, Derek, Le, Catherine H., Wen-Wei Liu, Ordinario, Ellen, Manohar, Chitra, Lee, Michael, Rajamani, Jaya, Huan Truong, Jing Li, Choi, Cindy, Jingchuan Li, Pati, Amrita, Bubendorf, Lukas, Buettner, Reinhard, Kerr, Keith M., Lopez-Rios, Fernando, Marchetti, Antonio, Marondel, Ivonne, Nicholson, Andrew G. |
| Source: |
Archives of Pathology & Laboratory Medicine. Jun2020, Vol. 144 Issue 6, p735-741. 7p. 1 Color Photograph, 1 Diagram, 4 Charts, 1 Graph. |
| Subjects: |
ADENOCARCINOMA, CELL lines, CYTOPLASM, IMMUNOHISTOCHEMISTRY, LUNG cancer, POLYMERASE chain reaction, PROTEIN-tyrosine kinases, FLUORESCENCE in situ hybridization, REVERSE transcriptase polymerase chain reaction, DESCRIPTIVE statistics, SEQUENCE analysis |
| Abstract: |
* Context.--The ability to determine ROS1 status has become mandatory for patients with lung adenocarcinoma, as many global authorities have approved crizotinib for patients with ROS1-positive lung adenocarcinoma. Objective.--To present analytical correlation of the VENTANA ROS1 (SP384) Rabbit Monoclonal Primary Antibody (ROS1 [SP384] antibody) with ROS1 fluorescence in situ hybridization (FISH). Design.--The immunohistochemistry (IHC) and FISH analytical comparison was assessed by using 122 non-- small cell lung cancer samples that had both FISH (46 positive and 76 negative cases) and IHC staining results available. In addition, reverse transcription--polymerase chain reaction (RT-PCR) as well as DNA and RNA nextgeneration sequencing (NGS) were used to further examine the ROS1 status in cases that were discrepant between FISH and IHC, based on staining in the cytoplasm of 2+ or above in more than 30% of total tumor cells considered as IHC positive. Here, we define the consensus status as the most frequent result across the 5 different methods (IHC, FISH, RT-PCR, RNA NGS, and DNA NGS) we used to determine ROS1 status in these cases. Results.--Of the IHC scoring methods examined, staining in the cytoplasm of 2+ or above in more than 30% of total tumor cells considered as IHC positive had the highest correlation with a FISH-positive status, reaching a positive percentage agreement of 97.8% and negative percentage agreement of 89.5%. A positive percentage agreement (100%) and negative percentage agreement (92.0%) was reached by comparing ROS1 (SP384) using a cutoff for staining in the cytoplasm of 2+ or above in more than 30% of total tumor cells to the consensus status. Conclusions.--Herein, we present a standardized staining protocol for ROS1 (SP384) and data that support the high correlation between ROS1 status and ROS1 (SP384) antibody. [ABSTRACT FROM AUTHOR] |
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| Database: |
Health Source: Nursing/Academic Edition |
