Phytochemical Analysis and Determination of the Chemical Composition of Larvicidal Extracts of Black Pepper ('Pipernigrum' L.) Waste: An Undergraduate Chemistry Experiment
| Title: | Phytochemical Analysis and Determination of the Chemical Composition of Larvicidal Extracts of Black Pepper ('Pipernigrum' L.) Waste: An Undergraduate Chemistry Experiment |
|---|---|
| Language: | English |
| Authors: | Lucas, Danielle R. (ORCID |
| Source: | Journal of Chemical Education. Apr 2021 98(4):1397-1403. |
| Availability: | Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc |
| Peer Reviewed: | Y |
| Page Count: | 7 |
| Publication Date: | 2021 |
| Document Type: | Journal Articles Reports - Descriptive |
| Education Level: | Higher Education Postsecondary Education |
| Descriptors: | Chemistry, Undergraduate Students, Science Experiments, Laboratory Experiments, Sanitation, Spectroscopy, Scientific Concepts, Graduate Students |
| DOI: | 10.1021/acs.jchemed.0c01106 |
| ISSN: | 0021-9584 |
| Abstract: | A laboratory experiment was developed to introduce undergraduate chemistry students to the chemical characterization of agroindustrial waste extracts by gas chromatography-mass spectrometry (GC--MS). The students prepared hexane and dichloromethane extracts from black pepper ("Piper nigrum" L.) waste. GC--MS analysis revealed the possible presence of sesquiterpenes and piperine, compounds commonly found in "P. nigrum" fruits. During postlab discussion, the students were encouraged to search for information about the biological properties of the putatively identified compounds. Most students found that piperine shows larvicidal activity. Thus, an experiment was conducted by a postgraduate research group to investigate the larvicidal potential of "P. nigrum" waste extracts. From the results, the students concluded that the hexane extract had higher larvicidal activity than the dichloromethane extract. We also provided students with the opportunity to identify piperine using (+)-APCI FT-ICR MS spectra. The proposed experiment helped students to learn and practice GC--MS and stimulated them to search the scientific literature for solutions to environmental problems. |
| Abstractor: | As Provided |
| Entry Date: | 2021 |
| Accession Number: | EJ1302536 |
| Database: | ERIC |
| ISSN: | 0021-9584 |
|---|---|
| DOI: | 10.1021/acs.jchemed.0c01106 |
| Published in: | Journal of Chemical Education |
| Language: | English |