Bibliographic Details
| Title: |
Pituitary Adenylate Cyclase Activating Peptide (1-38) and its analog (Acetyl-[Ala15, Ala20] PACAP 38-polyamide) reverse methacholine airway hyperresponsiveness in rats |
| Authors: |
Tlili, Mounira, Rouatbi, Sonia, Gandia, Fedoua, Hallegue, Dorsaf, Sriha, Badreddine, Yacoubi, Mohamed Taher, Krichah, Raja, Sakly, Mohsen, Rhouma, Khémais Ben, Vaudry, David, Wurtz, Olivier, Tebourbi, Olfa |
| Source: |
Brazilian Journal of Pharmaceutical Sciences. September 2015 51(3) |
| Publisher Information: |
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas, 2015. |
| Publication Year: |
2015 |
| Subject Terms: |
Pituitary adenylate cyclase activating peptide/effects/inflammatory lung diseases, Pituitary adenylate cyclase/analogs/effects in inflammatory lung diseases, Methacholine, Bronchodilators/effects, Inflammatory lung diseases/treatment |
| More Details: |
The aim of this study was to investigate both functionally and structurally bronchodilator effects of Pituitary adenylate cyclase activating peptide (PACAP38) and acetyl-[Ala15, Ala20] PACAP38-polyamide, a potent PACAP38 analog, in rats challenged by methacholine (MeCh). Male Wistar rats were divided randomly into five groups. Groups 1 and 2 inhaled respectively aerosols of saline or increasing doses of MeCh (0.5, 1, 2.12, 4.25, 8.5, 17, 34 and 68mg/L). The other groups received terbutaline (Terb) (250 µg/rat) (10-6 M), PACAP38 (50 µg/rat) (0.1 mM) or PACAP38 analog (50 µg/rat) associated to MeCh from the dose of 4.25 mg/L. Total lung resistances (RL) were recorded before and 2 min after MeCh administration by pneumomultitest equipment. MeCh administration induced a significant and a dose-dependent increase (p<0.05) of RL compared to control rats. Terb, PACAP38 and PACAP38 analog reversed significantly the MeCh-induced bronchial constriction, smooth muscle (SM) layer thickness and bronchial lumen mucus abundance. PACAP38 analog prevents effectively bronchial smooth muscle layer thickness, mucus hypersecretion and lumen decrease. Therefore, it may constitute a potent therapeutic bronchodilator. |
| Document Type: |
article |
| File Description: |
text/html |
| Language: |
English |
| ISSN: |
1984-8250 |
| DOI: |
10.1590/S1984-82502015000300020 |
| Access URL: |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300681 |
| Rights: |
info:eu-repo/semantics/openAccess |
| Accession Number: |
edssci.S1984.82502015000300681 |
| Database: |
SciELO |
