Bibliographic Details
| Title: |
Large-scale, high-efficiency production of coffee somatic embryos |
| Authors: |
Maciel, Anna Lygia de Rezende, Rodrigues, Filipe Almendagna, Pasqual, Moacir, Carvalho, Carlos Henrique Siqueira de |
| Source: |
Crop Breeding and Applied Biotechnology. June 2016 16(2) |
| Publisher Information: |
Crop Breeding and Applied Biotechnology, 2016. |
| Publication Year: |
2016 |
| Subject Terms: |
Vegetative propagation, biotechnology, tissue culture, Coffea arabica L |
| More Details: |
The study aims to compare the efficiency of previously used liquid media for coffee, evaluating NAA concentrations in liquid medium and proline in semi-solid medium in the regeneration of somatic embryos and asses concentrations of BA and IAA in the maturation of embryos in temporary immersion bioreactors. For the regeneration of globular embryos from embryogenic aggregates and calli, we tested five concentrations (0.00, 0.25, 0.50, 1.00 and 2.00 mg L-1) of NAA in liquid medium and five concentrations (0.0, 0.5, 1.0, 2.0 and 4.0 g L-1) of proline in semisolid medium. The multiplication of embryogenic aggregates was highest in culture medium MM, reaching a density 7.5 times greater than that of the initial density. NAA promoted a linear increase in embryo regeneration. The medium containing 2.0 mg L-1 BA and 0.0 mg L-1 IAA yielded the highest percentage of large cotyledonary embryos. |
| Document Type: |
article |
| File Description: |
text/html |
| Language: |
English |
| ISSN: |
1984-7033 |
| DOI: |
10.1590/1984-70332016v16n2a16 |
| Access URL: |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000200102 |
| Rights: |
info:eu-repo/semantics/openAccess |
| Accession Number: |
edssci.S1984.70332016000200102 |
| Database: |
SciELO |
