SeptiFast for diagnosis of sepsis in severely ill patients from a Brazilian hospital

Bibliographic Details
Title: SeptiFast for diagnosis of sepsis in severely ill patients from a Brazilian hospital
Authors: Sitnik, Roberta, Marra, Alexandre Rodrigues, Petroni, Roberta Cardoso, Ramos, Ozires Pereira Santos, Martino, Marinês Dalla Valle, Pasternak, Jacyr, Santos, Oscar Fernando Pavão dos, Mangueira, Cristóvão Luis Pitangueira, Pinho, João Renato Rebello
Source: einstein (São Paulo). June 2014 12(2)
Publisher Information: Instituto Israelita de Ensino e Pesquisa Albert Einstein, 2014.
Publication Year: 2014
Subject Terms: Sepsis/diagnosis, Multiplex polymerase chain reaction, Real-time polymerase chain reaction
More Details: Objective To test and validate a multiplex real-time polymerase chain reaction method for bloodstream infections, as well as to compare the results with conventional blood culture.Methods A total of 114 consecutive patients with clinical evidence of sepsis were submitted to blood culture and LightCycler™ SeptiFast tests.Results More positive specimens (23; 20.2%) were detected using the LightCycler™ SeptiFast than the blood culture (17; 14.9%), with an agreement of 86.8%. Discordant results were seen in four patients positive only to blood culture, ten positive only to LightCycler™ SeptiFast and one to different pathogens found by each test. Infections with microorganisms detected only using blood culture reassured the need to perform both tests. The mean time to results for blood culture was 5 days for negative and 3.5 days for positive results. LightCycler™ SeptiFast results were achieved in less than 8 hours.Conclusion LightCycler™ SeptiFast showed a high potential as a test to be carried out concomitantly with blood culture for sepsis diagnosis in severely ill patients. This test allowed a faster diagnosis of bacterial and fungal infections that helped to reduce hospital stay and to control the use of antibiotics. LightCycler™ SeptiFast can also eventually detect microorganism and infections that are hardly detected by blood culture, especiallyCandidanon-albicans infections.
Document Type: article
File Description: text/html
Language: English
ISSN: 1679-4508
DOI: 10.1590/S1679-45082014AO2932
Access URL: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082014000200191
Rights: info:eu-repo/semantics/openAccess
Accession Number: edssci.S1679.45082014000200191
Database: SciELO
More Details
ISSN:16794508
DOI:10.1590/S1679-45082014AO2932
Published in:einstein (São Paulo)
Language:English