Bibliographic Details
| Title: |
Overexpression of HIF-1α and Morphological Alterations in the Tongue of Rats Exposed To Secondhand Smoke |
| Authors: |
Amaral, Eleonora de Paula, Rosa, Rodrigo César, Etchebehere, Renata Margarida, Nogueira, Ruchele Dias, Volpon, José Batista, Rodrigues, Denise Bertulucci Rocha, Pereira, Sanívia Aparecida de Lima |
| Source: |
Brazilian Dental Journal. June 2020 31(3) |
| Publisher Information: |
Fundação Odontológica de Ribeirão Preto, 2020. |
| Publication Year: |
2020 |
| Subject Terms: |
rats, tobacco smoke, tongue |
| More Details: |
Smoking is a risk factor for serious health problems and is associated with several changes in the tissues of the oral cavity. The aim of this study was to evaluate the collagen percentage, mast cells density, intensity of immunolabeled cells by anti-HIF-1α in the musculature lingual of rats exposed to secondhand smoke. Twenty-seven female Wistar albino rats were divided into three groups: rats not exposed to tobacco smoke inhalation (Control group) (n=7); rats exposed to smoke inhalation for 30 days (TAB 30) (n=10); and rats exposed to smoke inhalation for 45 days (TAB 45) (n=10). Subsequently, the animals were submitted to euthanasia and removal of the tongue for histological and immunohistochemistry processing and analysis. In the groups TAB 30 and TAB 45 there were a lower percentage of collagen, a higher density of mast cells and a greater intensity of anti-HIF-1α immunolabeled cells compared to Control group. There was also a positive and significant correlation between the percentage of collagen and mast cell density. There was not significative difference between TAB 30 e TAB 45 in any of the parameters evaluated. Therefore, the exposure of rats to secondhand smoke for 45 days causes decrease in perimysial collagen fibers, increase in the number of mast cells and increase in the immunolabeling for HIF-1α in lingual muscle cells. The present study was the first to evaluate the percentage of collagen, mast cell density and immunostaining for HIF-1α in rat tongues exposed to tobacco smoke. |
| Document Type: |
article |
| File Description: |
text/html |
| Language: |
English |
| ISSN: |
0103-6440 |
| DOI: |
10.1590/0103-6440202002898 |
| Access URL: |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402020000300281 |
| Rights: |
info:eu-repo/semantics/openAccess |
| Accession Number: |
edssci.S0103.64402020000300281 |
| Database: |
SciELO |
