Bibliographic Details
| Title: |
Diversity of metallo-β-lactamase-encoding genes found in distinct species of Acinetobacter isolated from the Brazilian Amazon Region |
| Authors: |
Brasiliense, Danielle, Cayô, Rodrigo, Streling, Ana Paula, Nodari, Carolina S, Barata, Rafael R, Lemos, Poliana S, Massafra, Janaina M, Correa, Yan, Magalhães, Igor, Gales, Ana C, Sodré, Roberta |
| Source: |
Memórias do Instituto Oswaldo Cruz. January 2019 114 |
| Publisher Information: |
Instituto Oswaldo Cruz, Ministério da Saúde, 2019. |
| Publication Year: |
2019 |
| Subject Terms: |
metallo-β-lactamase, non-baumannii Acinetobacter species, polymyxin resistance, nosocomial infection, ICU, Tn125 |
| More Details: |
BACKGROUND The multidrug resistance (MDR) phenotype is frequently observed in Acinetobacter baumannii, the most clinically relevant pathogenic species of its genus; recently, other species belonging to the A. calcoaceticus-A. baumannii complex have emerged as important MDR nosocomial pathogens. OBJECTIVES The present study aimed to verify the occurrence of metallo-β-lactamase genes among distinct Acinetobacter species in a hospital located in the Brazilian Amazon Region. METHODS Antimicrobial susceptibility profiles were determined by broth microdilution. The genetic relationships among these isolates were assessed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Pyrosequencing reads of plasmids carrying the bla NDM-1 gene were generated using the Ion Torrent™ platform sequencing. FINDINGS A total of six isolates carried bla NDM-1: A. baumannii (n = 2), A. nosocomialis (n = 3), and A. pittii (n = 1); three carried bla IMP-1: A. baumannii, A. nosocomialis, and A. bereziniae. Resistance to colistin was observed for an NDM-1-producing A. nosocomialis isolate. Diverse PFGE patterns and sequence types were found among A. nosocomialis and A. baumannii isolates. The bla NDM-1 sequence was inserted in a Tn125 transposon, while the bla IMP-1 was found as a gene cassette of the class 1 integron In86. MAIN CONCLUSIONS To the best of our knowledge, this is the first report describing the dissemination of bla NDM-1 among distinct Acinetobacter species recovered from the same hospital in South America. |
| Document Type: |
article |
| File Description: |
text/html |
| Language: |
English |
| ISSN: |
0074-0276 |
| DOI: |
10.1590/0074-02760190020 |
| Access URL: |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100330 |
| Rights: |
info:eu-repo/semantics/openAccess |
| Accession Number: |
edssci.S0074.02762019000100330 |
| Database: |
SciELO |
