Food-poisoning bacteria detection carrier, and method for detecting food-poisoning bacteria
| Title: | Food-poisoning bacteria detection carrier, and method for detecting food-poisoning bacteria |
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| Patent Number: | 9,150,931 |
| Publication Date: | October 06, 2015 |
| Appl. No: | 13/696196 |
| Application Filed: | May 10, 2011 |
| Abstract: | A plurality of probes are immobilized on a carrier for detecting food poisoning bacteria, the plurality of probes being selected, either alone or in combination, respectively from two or more groups among a first probe group for detecting Escherichia coli, a second probe group for detecting Listeria, a third probe group for detecting Campylobacter, a fourth probe group for detecting Vibrio parahaemolyticus, a fifth probe group for detecting Staphylococcus aureus, a sixth probe group for detecting Salmonella, and a seventh probe group for detecting Bacillus cereus. Two or more types of food poisoning bacteria among Escherichia coli, Listeria, Campylobacter, Vibrio parahaemolyticus, Staphylococcus aureus, Salmonella, and Bacillus cereus can be specifically and simultaneously detected using the carrier. |
| Inventors: | Yamasaki, Takaaki (Yokohama, JP); Harada, Takaaki (Yokohama, JP); Saruwatari, Yoshihiro (Yokohama, JP); Kamei, Shuichi (Kudamatsu, JP) |
| Assignees: | TOYO SEIKAN GROUP HOLDINGS, LTD. (Tokyo, JP), Toyo Kohan Co., Ltd. (Tokyo, JP) |
| Claim: | 1. A microarray for detecting food poisoning bacteria, the microarray comprising: a plurality of probes immobilized on the microarray, wherein the plurality of probes comprises: a probe from a first probe group, a probe from a second probe group, a probe from a third probe group, a probe from a fourth probe group, a probe from a fifth probe group, a probe from a sixth probe group, and a probe from a seventh probe group, wherein the probe from the first probe group hybridizes to a nucleic acid fragment of Escherichia coli , and the first probe group consists of probes consisting of SEQ ID NO: 1 to 4 or the complements thereof, the probe from the second probe group hybridizes to a nucleic acid fragment of Listeria , and the second probe group consists of probes consisting of SEQ ID NO: 7 to 13 or the complements thereof, the probe from the third probe group hybridizes to a nucleic acid fragment of Campylobacter , and the third probe group consists of probes consisting of SEQ ID NO: 14 to 19 or the complements thereof, the probe from the fourth probe group hybridizes to a nucleic acid fragment of Vibrio parahaemolyticus , and the fourth probe group consists of probes consisting of SEQ ID NO: 20 to 24 or the complements thereof, the probe from the fifth probe group hybridizes to a nucleic acid fragment of Staphylococcus aureus , and the fifth probe group consists of probes consisting of SEQ ID NO: 25 to 29 or the complements thereof, the probe from the sixth probe group hybridizes to a nucleic acid fragment of Salmonella , and the sixth probe group consists of probes consisting of SEQ ID NO: 30 to 40 or the complements thereof, and the probe from the seventh probe group hybridizes to a nucleic acid fragment of Bacillus cereus , and the seventh probe group consists of probes consisting of SEQ ID NO: 41 to 45 or the complements thereof, wherein the plurality of probes specifically and simultaneously hybridize to the nucleic acid fragments of Escherichia coli, Listeria, Campylobacter, Vibrio parahaemolyticus, Staphylococcus aureus, Salmonella , and Bacillus cereus. |
| Claim: | 2. The microarray of claim 1 , wherein the plurality of probes comprises two probes selected respectively from each of all groups. |
| Patent References Cited: | 2001/0053519 December 2001 Fodor et al. 2010/0075305 March 2010 Ezaki et al. 2013/0059756 March 2013 Yamasaki et al. 101558168 October 2009 2005-034121 February 2005 2006-166912 June 2006 2008-200012 September 2008 2009-0027875 March 2009 WO 2008/041354 April 2008 |
| Other References: | Wang et al. Appl Microbiol Biotechnol 2007 vol. 76 pp. 225-233. cited by examiner PCT, “International Search Report and Written Opinion for PCT/JP2011/002592”, Jul. 19, 2011. cited by applicant Thompson FL., et al., “Phylogeny and molecular identification of vibrios on the basis of multilocus sequence analysis”, Applied and Environmental Microbiology, Sep. 2005, vol. 71. cited by applicant European Patent Office, “Extended European Search Report for EP 11780378.3”, Sep. 6, 2013. cited by applicant Suo B. et al., “Development of an oligonucleotide-based microarray to detect . . . ”, Molecular and Cellular Probes, Academic Press, London GB, vol. 24, No. 2, Apr. 1, 2010, p. 77-86. cited by applicant Lim Miao Chu et al., “Use of oligonucleotide array for . . . ”, Journal of Food Protection, Int'l Assn for Food Protection, US, vol. 68, No. 11, Nov. 1, 2005, p. 2278-2286. cited by applicant S.-Q. Jin et al., “Multiplexed Bead-Based Mesofluidic System for Detection of . . . ”, Applied and Environmental Microbiology, vol. 75, No. 21, Nov. 1, 2009, p. 6647-6654. cited by applicant Yiwen Liu-Stratton et al., “DNA Microarray technology in nutraceutical and food safety”, Toxicology Letters, vol. 150, No. 1, Apr. 1, 2004, p. 29-42. cited by applicant Fukushima, Hiroshi, “Simultaneous Multiplex Real-time SYBR Green PCR Analysis of 24 Target Genes of Food-Borne Bacteria”; Shimane Prefectural Institute of Public Health and Environment Science Ho, No. 50; Dec. 28, 2009; pp. 41-51. cited by applicant China Patent Office, “Office Action for 201180018595.8,” Oct. 31, 2013. cited by applicant Bang-Xing Hong et al., “Application of oligonucleotide array technology in rapid detection of common bacteria of foodborne diseases,” Chinese Journal of Laboratory Medicine, Feb. 2005, p. 169-172, vol. 28, No. 2, Department of Microbiology, School of Medicine, Sun Yatsen University, Guangzhou 510089, China. cited by applicant |
| Primary Examiner: | Haney, Amanda |
| Attorney, Agent or Firm: | Osha Liang LLP |
| Accession Number: | edspgr.09150931 |
| Database: | USPTO Patent Grants |
| Language: | English |
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