Spectrophotometric method for determining the viability of a sample containing platelets
| Title: | Spectrophotometric method for determining the viability of a sample containing platelets |
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| Patent Number: | 6,984,526 |
| Publication Date: | January 10, 2006 |
| Appl. No: | 09/861781 |
| Application Filed: | May 21, 2001 |
| Abstract: | A method and apparatus for characterizing the type of a blood sample and a variety of blood components are provided wherein a transmission spectrum of the sample is collected over a predetermined wavelength range. For blood typing, this spectrum is then compared with a set of control spectra collected from control blood samples having known blood types, from which the type of the blood sample can be determined. Further methods and apparatus are provided for determining the viability of and for cross matching a platelet unit. Additional method and apparatus permit analysis of the sample for the presence of a contaminant. Particles can also be counted in the sample, even when present in low concentrations, including white blood cell. |
| Inventors: | Garcia-Rubio, Luis Humberto (Temple Terrace, FL, US); Potter, Robert (Tampa, FL, US); Leparc, German (Tampa, FL, US); Orton, Sharyn (New Port Richey, FL, US); Mattley, Yvette (Tampa, FL, US); Bacon, Christina (Tampa, FL, US) |
| Assignees: | University of South Florida (Tampa, FL, US) |
| Claim: | 1. A method for determining the platelet viability of a test fluid sample containing platelets comprising the steps of: obtaining a test fluid sample containing platelets; collecting a multiwavelength transmission spectra of the test fluid sample over a predetermined wavelength range, wherein the wavelength range further comprises wavelengths in the ultra violet spectrum; obtaining a control fluid sample known to contain viable platelets; collecting a multiwavelength transmission spectra of the control fluid sample over a predetermined wavelength range, wherein the wavelength range further comprises wavelengths in the ultra violet spectrum; comparing the test fluid sample transmission spectra with the transmission spectra of the control fluid sample; and determining the platelet viability of the test fluid sample by comparing the quantitative spectral differences between the test fluid sample and the control fluid sample. |
| Claim: | 2. The method recited in claim 1 , further comprising prior to the step of collecting a transmission spectra of the test fluid sample, the steps of: diluting the test fluid sample to a predetermined concentration of platelets in the sample; and diluting the control fluid sample to the predetermined concentration of platelets in the control sample. |
| Claim: | 3. The method recited in claim 1 , wherein the range comprises approximately 190 to 820 nm. |
| Claim: | 4. The method recited in claim 1 , further comprising prior to the comparing step, the step of: normalizing the transmission spectra for the test fluid sample and the transmission spectra for the control fluid sample to an extinction coefficient at a predetermined normalized wavelength. |
| Claim: | 5. The method recited in claim 4 , wherein the predetermined normalized wavelength comprises approximately 280 nm. |
| Claim: | 6. The method recited in claim 1 , wherein the test fluid sample is contained in a translucent delivery bag and the control fluid sample is contained in a translucent delivery bag. |
| Current U.S. Class: | 436/171 |
| Patent References Cited: | 4213764 July 1980 O'Connor 4446239 May 1984 Tsuji et al. 4682887 July 1987 Bellhouse et al. 4988630 January 1991 Chen et al. 5110726 May 1992 Ogden 5180661 January 1993 Brubaker 5325295 June 1994 Fratantoni et al. 5336597 August 1994 McMillan 5709991 January 1998 Lin et al. 5871900 February 1999 Wollowitz et al. 0 353 206 July 1989 0 327 093 October 1990 |
| Other References: | Garcia-Rubio, L. H., The Effect of Molecular Size on the Absorption Spectra of Macromolecules, Macromolecules, vol. 20, No. 12, 1987. cited by other |
| Assistant Examiner: | Gabel, Gailene R. |
| Primary Examiner: | Le, Long V. |
| Attorney, Agent or Firm: | Sauter, Molly L. Smith & Hopen, P.A. |
| Accession Number: | edspgr.06984526 |
| Database: | USPTO Patent Grants |
| Language: | English |
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