Physalia fluorescent proteins
| Title: | Physalia fluorescent proteins |
|---|---|
| Patent Number: | 6,900,289 |
| Publication Date: | May 31, 2005 |
| Appl. No: | 10/226877 |
| Application Filed: | August 22, 2002 |
| Abstract: | Provided are Physalia fluorescent proteins (PFPs) and, more particularly, to PFPs of a Physalia species, and methods of detecting and isolating PFPs. Also provided are methods and compositions for using PFPs, including recombinant PFPs, as reporter molecules in in vitro and in vivo biological assays, including screening assays and cellular assays. |
| Inventors: | Yanagihara, Angel Anne (Honolulu, HI, US) |
| Assignees: | The University of Hawaii (Honolulu, HI, US) |
| Claim: | 1. An isolated Physalia fluorescent protein (PFP) characterized by an emission wavelength of about 456 nm, when said PFP is excited at an excitation wavelength of 221 nm. |
| Claim: | 2. An isolated Physalia fluorescent protein (PFP) characterized by an emission wavelength of about 421 nm, when said PFP is excited at an excitation wavelength of 222 nm. |
| Claim: | 3. An isolated Physalia fluorescent protein (PFP) characterized by an emission wavelength of about 600 nm, when said PFP excited at an excitation wavelength of 222 nm. |
| Claim: | 4. An isolated Physalia fluorescent protein (PFP) characterized by an emission wavelength of about 597 nm, when said PFP is excited at an excitation wavelength of 301 nm. |
| Claim: | 5. An isolated Physalia fluorescent protein (PFP) characterized by an emission wavelength of about 598 nm, when said PFP is excited at an excitation wavelength of 301 nm. |
| Claim: | 6. An isolated Physalia fluorescent protein (PFP) characterized by an emission wavelength of about 600 nm, when said PFP is excited at an excitation wavelength of 301 nm. |
| Claim: | 7. An isolated Physalia fluorescent protein (PFP) characterized by an emission wavelength in a range of about 888-898 nm, when said PFP is excited at an excitation wavelength of 301 nm. |
| Claim: | 8. An isolated Physalia fluorescent protein (PFP) characterized by an emission wavelength of about 888 nm, when said PFP is excited at an excitation wavelength of 301 nm. |
| Claim: | 9. An isolated Physalia fluorescent protein (PFP) characterized by an emission wavelength of about 838 nm, when said PFP is excited at an excitation wavelength of 421 nm. |
| Claim: | 10. An isolated Physalia fluorescent protein (PFP) characterized by an emission wavelength of about 900 nm, when said PFP is excited at an excitation wavelength of 450 nm. |
| Claim: | 11. An isolated Physalia fluorescent protein (PFP) characterized by an emission wavelength of about 898 nm, when said PFP is excited at an excitation wavelength of 451 nm. |
| Claim: | 12. The Physalia fluorescent protein (PFP) of any one of claims 1 - 11 , wherein said protein is further characterized by a molecular weight of about 10-15 kDa. |
| Claim: | 13. The Physalia fluorescent protein (PFP) of any one of claims 1 - 11 , wherein said protein is further characterized by a molecular weight of about 35-40 kDa. |
| Claim: | 14. The Physalia fluorescent protein (PFP) of any one of claims 1 - 11 , wherein said protein is of the species P. utriculus. |
| Current U.S. Class: | 530/350 |
| Patent References Cited: | 4471470 September 1984 Swainson et al. 5325324 June 1994 Rentzepis et al. 5625048 April 1997 Tsien et al. 6046925 April 2000 Tsien et al. 6124128 September 2000 Tsien et al. WO 96/23810 August 1996 |
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| Assistant Examiner: | Mondesi, Robert B. |
| Primary Examiner: | Wax, Robert A. |
| Attorney, Agent or Firm: | Dorsey & Whitney LLP Lorenz, Todd A. Ropp, Traci H. |
| Accession Number: | edspgr.06900289 |
| Database: | USPTO Patent Grants |
| Language: | English |
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