Method for regulating cold and dehydration regulatory genes in a plant
| Title: | Method for regulating cold and dehydration regulatory genes in a plant |
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| Patent Number: | 5,891,859 |
| Publication Date: | April 06, 1999 |
| Appl. No: | 08/949,603 |
| Application Filed: | October 14, 1997 |
| Abstract: | A gene, designated as CBF1, encoding a protein, CBF1, which binds to a region regulating expression of genes which promote cold temperature and dehydration tolerance in plants is described. CBF1 is used to transform microorganisms and can be used to transform plants. |
| Inventors: | Thomashow, Michael F. (East Lansing, MI); Stockinger, Eric J. (East Lansing, MI); Jaglo-Ottosen, Kirsten (Lansing, MI); Zarka, Daniel (Lansing, MI); Gilmour, Sarah Jane (Leslie, MI) |
| Assignees: | Michigan State University (East Lansing, MI) |
| Claim: | We claim |
| Claim: | 1. A method for regulating cold and dehydration regulatory genes in a plant comprising |
| Claim: | transforming a plant with a gene encoding a binding protein comprising an amino acid sequence sufficiently homologous to SEQ. ID. No. 2 that the protein is capable of selectively binding to a DNA regulatory sequence comprising CCG in the plant which regulates expression of one or more environmental stress tolerance genes in the plant; and |
| Claim: | expressing the binding protein in the plant. |
| Claim: | 2. The method according to claim 1 wherein the plant has a cold or dehydration transcription regulating region comprising a sequence CCGAC. |
| Claim: | 3. The method according to claim 1 wherein the plant has a cold or dehydration transcription regulating region comprising a sequence selected from the group consisting of SEQ. ID. Nos. 3, 7, 8, and 9. |
| Claim: | 4. The method according to claim 1 wherein the plant has a cold or dehydration transcription regulating region of Arabidopsis thaliana or a homologous regulatory region of another plant species. |
| Claim: | 5. The method according to claim 1 wherein the gene transformed into the plant comprises a DNA sequence as set forth in nucleotides 159 to 797 of SEQ ID No:1. |
| Claim: | 6. The method according to claim 1 wherein the binding protein comprises the amino acid sequence set forth in SEQ. ID. No. 2. |
| Claim: | 7. The method according to claim 1 wherein the plant is a crop or horticultural plant. |
| Claim: | 8. A method for regulating cold and dehydration regulatory genes in a plant comprising |
| Claim: | transfomning a plant with a gene encoding a binding protein comprising an amino acid sequence sufficiently homologous to an AP2 domain of SEQ. ID. No. 2 that the protein is capable of selectively binding to a DNA regulatory sequence comprising CCG in the plant which regulates expression of one or more environmental stress tolerance genes in the plant; and |
| Claim: | 9. The method according to claim 8 wherein the plant has a cold or dehydration transcription regulating region comprising a sequence CCGAC. |
| Claim: | 10. The method according to claim 8 wherein the plant has a cold or dehydration transcription regulating region comprising a sequence selected from the group consisting of SEQ. ID. Nos. 3, 7, 8, and 9. |
| Claim: | 11. The method according to claim 8 wherein the plant has a cold or dehydration transcription regulating region of Arabidopsis thaliana or a homologous regulatory region of another plant species. |
| Claim: | 12. The method according to claim 8 wherein the plant is a crop or horticultural plant. |
| Claim: | 13. A method for regulating cold and dehydration regulatory genes in a plant comprising |
| Claim: | contacting the plant with a composition comprising a binding protein comprising an amino acid sequence sufficiently homologous to SEQ. ID. No. 2 that the protein is capable of selectively binding to a DNA regulatory sequence comprising CCG in the plant which regulates expression of one or more environmental stress tolerance genes in the plant; |
| Claim: | having the binding protein enter the plant and regulate the expression of one or more environmental stress tolerance genes. |
| Claim: | 14. The method according to claim 13 wherein the composition is substantially free from other proteins. |
| Claim: | 15. The method according to claim 13 wherein the plant has a cold or dehydration transcription regulating region which comprises a sequence CCGAC. |
| Claim: | 16. The method according to claim 13 wherein the plant has a cold or dehydration transcription regulating region which comprises a sequence selected from the group consisting of SEQ. ID. Nos. 3, 7, 8, and 9. |
| Claim: | 17. The method according to claim 13 wherein the plant has a cold or dehydration transcription regulating region of Arabidopsis thaliana or a homologous regulatory region of another plant species. |
| Claim: | 18. The method according to claim 13 wherein the binding protein comprises the amino acid sequence set forth in SEQ. ID. No. 2. |
| Claim: | 19. The method according to claim 13 wherein the composition further comprises a carrier for introducing a protein into a plant cells. |
| Claim: | 20. The method according to claim 13 wherein the plant is a crop or horticultural plant. |
| Current U.S. Class: | 514/44; 530/350; 530/370; 530/379; 536/236; 435/440; 800/278 |
| Current International Class: | C07H 2104; C07K 100 |
| Patent References Cited: | 5296462 March 1994 Thomashow 5356816 October 1994 Thomashow |
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| Primary Examiner: | Marschel, Ardin H. |
| Assistant Examiner: | Riley, Jezia |
| Attorney, Agent or Firm: | Wilson Sonsini Goodrich & Rosati |
| Accession Number: | edspgr.05891859 |
| Database: | USPTO Patent Grants |
| Language: | English |
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