Bibliographic Details
| Title: |
METHODS FOR GENERATING CARTILAGE TISSUE |
| Document Number: |
20110313541 |
| Publication Date: |
December 22, 2011 |
| Appl. No: |
12/516859 |
| Application Filed: |
November 29, 2007 |
| Abstract: |
A hyaline-like, single layer cartilage tissue construct includes chondrogenic cells dispersed within an endogenously produced extracellular matrix. The single layer cartilage tissue construct has a glycosaminoglycan content substantially equal to the glycosaminoglycan content of native cartilage tissue. A method for generating a single layer cartilage tissue construct includes isolating a population of chondrogenic cells and then expanding the population of chondrogenic cells. Next, the population of chondrogenic cells is seeded into a bioreactor having a volume defined by oppositely disposed gas permeable membranes. The population of chondrogenic cells is then cultured in a serum-free culture medium for a time sufficient to permit the population of chondrogenic cells to differentiate and form the single layer cartilage tissue construct. |
| Inventors: |
Dennis, James E. (Cleveland Heights, OH, US); Awadallah, Amad A. (Cleveland Heights, OH, US); Caplan, Amold I. (Cleveland Heights, OH, US); Weidenbecher, Mark (Cleveland, OH, US) |
| Claim: |
1-12. (canceled) |
| Claim: |
13. A hyaline-like, multilayer cartilage tissue construct comprising a plurality of layers having a glycosaminoglycan content substantially equal to the glycosaminoglycan content of native cartilage tissue, each of the layers comprising chondrogenic cells dispersed within an endogenously produced extracellular matrix. |
| Claim: |
14. The multilayer cartilage tissue construct of claim 13, the chondrogenic cells being autogenic, allogenic, or a combination thereof. |
| Claim: |
15. The multilayer cartilage tissue construct of claim 13, the chondrogenic cells being immature chondrocytes, mature chondrocytes, or a combination thereof. |
| Claim: |
16. The multilayer cartilage tissue construct of claim 13, the multilayer cartilage tissue construct having a thickness of about 1 mm to about 4 mm. |
| Claim: |
17. A method for generating a multilayer cartilage tissue construct, the method comprising the steps of: isolating a population of chondrogenic cells; expanding the population of chondrogenic cells; seeding the population of chondrogenic cells into a bioreactor having a volume defined by oppositely disposed gas permeable membranes, the bioreactor including a serum-free culture medium; culturing the population of chondrogenic cells in the bioreactor for a time sufficient to permit the population of chondrogenic cells to differentiate and form a single layer cartilage tissue construct; removing the single layer cartilage tissue construct from the bioreactor; and forming the single layer cartilage tissue construct into the multilayer cartilage tissue construct. |
| Claim: |
18. The method of claim 17, the step of forming the single layer cartilage tissue construct into the multilayer cartilage construct further comprising folding a first portion of the single layer cartilage tissue construct onto a second portion of the single layer cartilage tissue construct and then applying hydrostatic pressure, mechanical pressure, or a combination thereof to form the multilayer cartilage tissue construct. |
| Claim: |
19. The method of claim 17, the step of forming the single layer cartilage tissue construct into the multilayer cartilage construct further comprising overlaying at least one single layer cartilage tissue construct onto at least one other single layer cartilage tissue construct and then applying hydrostatic pressure, mechanical pressure, or a combination thereof to form the multilayer cartilage tissue construct. |
| Claim: |
20. The method of claim 17, the population of chondrogenic cells comprising immature chondrocytes, mature chondrocytes, or a combination thereof. |
| Claim: |
21. The method of claim 17, the population of chondrogenic cells being autogenic, allogenic, or a combination thereof. |
| Claim: |
22. The method of claim 17, the step of expanding the population of chondrogenic cells further comprising culturing the population of chondrogenic cells in a conditioned growth media effective to promote expansion of the population of chondrogenic cells. |
| Claim: |
23. The method of claim 17, the serum-free culture medium including at least one growth factor selected from the group consisting of transforming growth factor-β, platelet-derived growth factor, insulin-like growth factor, acid fibroblast growth factor, basic fibroblast growth factor, epidermal growth factor, hepatocytic growth factor, keratinocyte growth factor, and bone morphogenic protein. |
| Claim: |
24. The method of claim 17, the population of chondrogenic cells endogenously producing an extracellular matrix when the population of chondrogenic cells is cultured in the bioreactor. |
| Claim: |
25. The method of claim 17, the step of culturing the population of chondrogenic cells in the bioreactor further comprising growing the population of chondrogenic cells at about 37° C. in a humidified atmosphere with the addition of about 5% carbon dioxide and about 1% to about 21% oxygen. |
| Claim: |
26. A multilayer cartilage tissue construct produced by a method of claim 17. |
| Claim: |
27. A method for generating a tracheal implant, the method comprising the steps of: isolating a population of chondrogenic cells; expanding the population of chondrogenic cells; seeding the population of chondrogenic cells into a bioreactor having a volume defined by oppositely disposed gas permeable membranes, the bioreactor including a serum-free culture medium; culturing the population of chondrogenic cells in the bioreactor for a time sufficient to permit the population of chondrogenic cells to differentiate and form a single layer cartilage tissue construct; removing the single layer cartilage tissue construct from the bioreactor; and forming the tracheal implant with the single layer cartilage tissue construct. |
| Claim: |
28. The method of claim 27, the tracheal implant comprising a whole trachea. |
| Claim: |
29. The method of claim 28, the tracheal implant comprising a portion of the whole trachea. |
| Claim: |
30-33. (canceled) |
| Current U.S. Class: |
623/2/372 |
| Current International Class: |
61; 12 |
| Accession Number: |
edspap.20110313541 |
| Database: |
USPTO Patent Applications |
