Bibliographic Details
| Title: |
STOOL SAMPLE PROCESSING METHOD AND STOOL SAMPLE PROCESSING CONTAINER |
| Document Number: |
20110060137 |
| Publication Date: |
March 10, 2011 |
| Appl. No: |
12/944260 |
| Application Filed: |
November 11, 2010 |
| Abstract: |
The present invention relates to a stool sample processing method and stool sample processing container the a stool sample processing container provided with a stool collection tool, a suspending solution holding portion and a processing solution holding portion, wherein stool sample preparation solutions consisting of a suspending solution and a stool sample processing solution are respectively housed in a suspending solution holding container and a processing solution holding container, after first mixing a stool sample with the suspending solution and suspending therein, a sealant is released into the suspending solution holding container by pressing on the lower portion of the processing solution holding container, and the resulting stool suspension mixes with the stool sample processing solution that stabilizes the nucleic acid. |
| Inventors: |
Tanigami, Yasuo (Tokyo, JP); Nakajima, Kazue (Tokyo, JP); Shibazaki, Takami (Tokyo, JP) |
| Assignees: |
OLYMPUS CORPORATION (Tokyo, JP) |
| Claim: |
1. A stool sample processing method for preparing and processing a stool sample for recovering a nucleic acid from the collected stool sample, comprising: forming a stool suspension in which the collected stool sample is suspended with a suspending solution, and preparing the stool sample by mixing the stool suspension with a stool sample processing solution that stabilizes the nucleic acid. |
| Claim: |
2. The stool sample processing method according to claim 1, wherein the suspending solution is selected from the group consisting of water, physiological saline or a buffer. |
| Claim: |
3. The stool sample processing method according to claim 1, wherein the stool sample processing solution is a water-soluble organic solvent. |
| Claim: |
4. The stool sample processing method according to claim 3, wherein the water-soluble organic solvent is a water-soluble alcohol and/or ketone. |
| Claim: |
5. The stool sample processing method according to claim 3, wherein the stool sample contains 30% or more of the water-soluble organic solvent. |
| Claim: |
6. The stool sample processing method according to claim 4, wherein the water-soluble alcohol is one or more types selected from the group consisting of ethanol, propanol and methanol. |
| Claim: |
7. The stool sample processing method according to claim 4, wherein the ketone is acetone and/or methyl ethyl ketone. |
| Claim: |
8. The stool sample processing method according to claim 3, wherein the water-soluble organic solvent is an aldehyde. |
| Claim: |
9. The stool sample processing method according to claim 3, wherein the stool sample contains 0.01 to 30% of the water-soluble organic solvent. |
| Claim: |
10-11. (canceled) |
| Claim: |
12. The stool sample processing method according to claim 3, wherein the water-soluble organic solvent contains an organic acid. |
| Claim: |
13. The stool sample processing method according to claim 3, wherein the water-soluble organic solvent contains a chelating agent and/or polycation. |
| Claim: |
14. A stool sample processing container for preparing and processing a stool sample for recovering a nucleic acid from collected stool sample, comprising: a suspending solution holding portion that holds a suspending solution for suspending the stool sample, an introduction mechanism that introduces the stool sample into the suspending solution holding portion, a processing solution holding portion that holds a stool sample processing solution that stabilizes the nucleic acid, and a release mechanism that releases an opening between the suspending solution holding portion and the processing solution holding portion. |
| Claim: |
15. The stool sample processing container according to claim 14, wherein the release mechanism is provided with a communicating hole that connects the suspending solution holding portion and the processing solution holding portion, and a sealing portion provided in the communication hole that seals communication; wherein, the processing solution holding portion is a flexible container, and the stool sample processing solution is allowed to flow towards the suspending solution by cancelling sealing of the sealing portion by increasing pressure of the processing solution by contracting the processing solution holding container. |
| Claim: |
16. The stool sample processing container according to claim 14, wherein the processing solution holding portion is flexible in the axial direction, and allows all of the stool sample processing solution to flow towards the suspending solution holding portion. |
| Claim: |
17. The stool sample processing container according to claim 14, wherein the release mechanism is provided with a sealing portion that seals an opening between the suspending solution holding portion and the processing solution holding portion, and a protruding portion that releases the opening between the suspending solution holding portion and the processing solution holding portion by puncturing the sealing portion. |
| Claim: |
18. The stool sample processing container according to claim 14, wherein the release mechanism is provided with a sealing portion that seals an opening between the suspending solution holding portion and the processing solution holding portion, and a protruding portion that releases the opening between the suspending solution holding portion and the processing solution holding portion by pressing on the sealing portion. |
| Claim: |
19. The stool sample processing container according to claim 14, wherein the processing solution holding portion is formed within the suspending solution holding portion and the suspending solution holding portion and the processing solution holding portion are formed with an elastic material, and the stool sample processing solution is allowed to flow towards the suspending solution by rupturing the processing solution holding portion by pressing from outside the suspending solution holding portion and the processing solution holding portion. |
| Claim: |
20. The stool sample processing container according to claim 14, wherein the processing solution holding portion is formed within the suspending solution holding portion, the suspending solution holding portion and the processing solution holding portion are formed with an elastic material, the tensile strength of a portion of the processing solution holding portion is lower than the tensile strength of the suspending solution holding portion, and the stool sample processing solution is allowed to flow toward the suspending solution by rupturing the portion of weak tensile strength of the processing solution holding portion by bending the suspending solution holding portion and the processing solution holding portion from outside the suspending solution holding portion and the processing solution holding portion. |
| Claim: |
21. The stool sample processing container according to claim 14, wherein at least the suspending solution holding portion and the processing solution holding portion are separate portions, and the suspending solution holding portion and the processing solution holding portion are mutually attachable. |
| Claim: |
22. The stool sample processing container according to claim 14, wherein the suspending solution is selected from the group consisting of water, physiological saline or a buffer. |
| Claim: |
23. The stool sample processing container according to claim 14, wherein the stool sample processing solution is a water-soluble organic solvent. |
| Claim: |
24. The stool sample processing container according to claim 23, wherein the water-soluble organic solvent is a water-soluble alcohol and/or ketone. |
| Claim: |
25. The stool sample processing container according to claim 23, wherein the stool sample contains 30% or more of the water-soluble organic solvent. |
| Claim: |
26. The stool sample processing container according to claim 24, wherein the water-soluble alcohol is one or more types selected from the group consisting of ethanol, propanol and methanol. |
| Claim: |
27. The stool sample processing container according to claim 24, wherein the ketone is acetone and/or methyl ethyl ketone. |
| Claim: |
28. The stool sample processing container according to claim 23, wherein the water-soluble organic solvent is an aldehyde. |
| Claim: |
29. The stool sample processing container according to claim 25, wherein the stool sample contains 0.01 to 30% of the water-soluble organic solvent. |
| Claim: |
30-31. (canceled) |
| Claim: |
32. The stool sample processing container according to claim 23 wherein, the water-soluble organic solvent contains an organic acid. |
| Claim: |
33. The stool sample processing container according to claim 23, wherein the water-soluble organic solvent contains a chelating agent and/or polycation. |
| Current U.S. Class: |
536/231 |
| Current International Class: |
07; 01 |
| Accession Number: |
edspap.20110060137 |
| Database: |
USPTO Patent Applications |
