| Title: |
Nucleic Acids Encoding a Functional Mammalin Purinoreceptor, P2X3, Methods of Production and Use Thereof |
| Document Number: |
20100221773 |
| Publication Date: |
September 2, 2010 |
| Appl. No: |
11/992634 |
| Application Filed: |
September 26, 2006 |
| Abstract: |
The subject invention relates to the rhesus monkey P2X3 receptor, nucleic acids encoding this receptor, methods of modulating the activity of a target purinergic receptor using the P2X3 receptor and to uses of these methods. In particular, such methods may be used, for example, to accelerate the rate of resensitization of a desensitized receptor. |
| Inventors: |
Kane, Stefanie A. (Schwenksville, PA, US); Mallee, John J. (Collegeville, PA, US); Salvatore, Christopher (Philadelphia, PA, US); Wang, Jixin (Wayne, PA, US) |
| Claim: |
1.-15. (canceled) |
| Claim: |
16. An isolated polynucleotide encoding a rhesus P2X3 receptor protein comprising the amino acid sequence of SEQ ID NO.:2. |
| Claim: |
17. An isolated polynucleotide encoding a receptor protein of claim 16, or a functionally active fragment thereof, wherein said polynucleotide comprises a sequence selected from the group consisting of: (a) a sequence of nucleotides that encodes the amino acid sequence set forth in SEQ ID NO.:2, or a functionally active fragment thereof; (b) a sequence of nucleotides as set forth in SEQ ID NO.:1; and (c) a sequence of nucleotides that encode a mammalian purinergic receptor subtype and that hybridizes to the sequence of nucleotides of (a) or (b) under moderately stringent conditions, wherein said sequence of nucleotides encodes a biologically active mammalian purinergic receptor subtype designated as P2X3 herein. |
| Claim: |
18. An expression vector comprising a polynucleotide of claim 17. |
| Claim: |
19. A host cell transformed or transfected with the expression vector of claim 18. |
| Claim: |
20. A host cell of claim 19, wherein said cell is selected from the group consisting of a bacterial cell, a mammalian cell, a yeast cell and an amphibian cell. |
| Claim: |
21. A host cell according to claim 20, wherein the cell is an amphibian cell. |
| Claim: |
22. A host cell according to claim 20, wherein the cell is a mammalian cell. |
| Claim: |
23. A method for producing a rhesus P2X3 receptor polypeptide, the method comprising the steps of: (a) transfecting or transforming a suitable host cell with the expression vector of claim 18; (b) culturing the host cells of step (a) under conditions which allow expression of the recombinant receptor polypeptide from the expression vector; and (c) harvesting the recombinant receptor polypeptide from the culture, culturing a host. |
| Current U.S. Class: |
435/691 |
| Current International Class: |
12; 07; 12; 12; 12; 12 |
| Accession Number: |
edspap.20100221773 |
| Database: |
USPTO Patent Applications |
