Bibliographic Details
| Title: |
CULTURE MEDIUM FOR GINGIVAL FIBROBLASTS |
| Document Number: |
20090061512 |
| Publication Date: |
March 5, 2009 |
| Appl. No: |
12/051559 |
| Application Filed: |
March 19, 2008 |
| Abstract: |
The present invention relates to a gingival fibroblast culture medium free of animal serum, comprising an animal cell culture medium, free of animal serum, to which is added: from 0.1 ng/ml to 100 ng/ml bFGF, and/or from 1 μg/ml to 50 μg/ml insulin. |
| Inventors: |
GOGLY, Bruno (Hondevilliers, FR); COULOMB, Bernard (IGNY, FR); LAFONT, Antoine (Paris, FR) |
| Claim: |
1. A gingival fibroblast culture medium free of animal serum, comprising a culture medium for animal or human cells, free of animal serum, to which is added: from 0.1 ng/ml to 100 ng/ml bFGF, and/or from 1 μg/ml to 50 μg/ml insulin. |
| Claim: |
2. The gingival fibroblast culture medium of claim 1, wherein bFGF is added at 1 ng/ml. |
| Claim: |
3. The gingival fibroblast culture medium of claim 1, wherein insulin is added at 5 μg/ml. |
| Claim: |
4. The gingival fibroblast culture medium according to claim 1, wherein the animal cell culture medium comprises 8 volumes DMEM and 2 volumes KNOCKOUT™ SR (INVITROGEN) or 9 volumes DMEM and 1 volume KNOCKOUT™ SR (INVITROGEN). |
| Claim: |
5. The gingival fibroblast culture medium according to claim 1, comprising bFGF at 1 ng/ml and insulin at 15 μg/ml. |
| Claim: |
6. The gingival fibroblast culture medium according to claim 1, further comprising platelet lysate. |
| Claim: |
7. The use of a gingival fibroblast culture as defined in claim 1, for the culture of gingival fibroblast. |
| Claim: |
8. A method for the culture of gingival fibroblasts which comprises growing cells in a gingival fibroblast culture as defined in claim 1. |
| Claim: |
9. A kit for cultivating gingival fibroblasts, comprising: a gingival fibroblast culture medium as defined in claim 1, and platelet lysate. |
| Current U.S. Class: |
435/366 |
| Current International Class: |
12 |
| Accession Number: |
edspap.20090061512 |
| Database: |
USPTO Patent Applications |
