Bibliographic Details
| Title: |
Modified Sarcosine Oxidases, Genes and Recombinant DNAs Thereof, and Methods for Preparing The Same |
| Document Number: |
20070269860 |
| Publication Date: |
November 22, 2007 |
| Appl. No: |
11/627484 |
| Application Filed: |
January 26, 2007 |
| Abstract: |
(1) A modified sarcosine oxidase with improved stability in the acidic range compared to a wild-type sarcosine oxidase. (2) A sarcosine oxidase gene encoding a modified sarcosine oxidase of the following (a), (b), or (c): (a) protein composed of the amino acid sequence represented by SEQ ID NO: 1 (b) protein composed of an amino acid sequence wherein one or some amino acid(s) are deleted, substituted, or added from the amino acid sequence represented by SEQ ID NO: 1, and which has sarcosine oxidase activity (c) protein composed of an amino acid sequence which shows 80% or more homology to the amino acid sequence represented by SEQ ID NO: 1, and which has sarcosine oxidase activity According to the present invention, sarcosine oxidases, in particular sarcosine oxidases which show optimal pH and high activity in the slightly acidic range and have improved stability can be prepared efficiently, thus making the invention industrially useful. |
| Inventors: |
FURUKAWA, Keisuke (Chiba, JP); Kajiyama, Naoki (Chiba, JP) |
| Assignees: |
Kikkoman Corporation (Noda-shi, JP) |
| Claim: |
1-6. (canceled) |
| Claim: |
7. Sarcosine oxidase genes encoding modified sarcosine oxidases of the following (a), (b), or (c): (a) protein composed of the amino acid sequence represented by SEQ ID NO: 1; (b) protein composed of an amino acid sequence wherein one or some amino acid(s) are deleted, substituted, or added from the amino acid sequence represented by SEQ ID NO: 1, and which have sarcosine oxidase activity; or (c) protein composed of an amino acid sequence which shows 80% or more homology to the amino acid sequence represented by SEQ ID NO: 1, and which has sarcosine oxidase activity. |
| Claim: |
8. A recombinant DNA characterized in that the sarcosine oxidase gene according to claim 7 is inserted into a vector DNA. |
| Claim: |
9. A transformant or transductant comprising the recombinant DNA according to claim 8. |
| Claim: |
10. A method for preparing a modified sarcosine oxidase characterized by culturing the transformant or transductant according to claim 9 in a medium, and collecting a sarcosine oxidase from the culture. |
| Current U.S. Class: |
435069/100 |
| Current International Class: |
07 |
| Accession Number: |
edspap.20070269860 |
| Database: |
USPTO Patent Applications |
