Bibliographic Details
| Title: |
Analytical Evaluation of Dried Blood Spot and Rapid Diagnostic Test as a New Strategy for Serological Community Screening for Chronic Chagas Disease |
| Authors: |
Aroa Silgado, Lídia Gual-Gonzalez, Adrián Sánchez-Montalvá, Inés Oliveira-Souto, Lidia Goterris, Nuria Serre-Delcor, Juliana Esperalba, Jordi Gomez-i-Prat, Candela Fernández-Naval, Israel Molina, Tomas Pumarola, Elena Sulleiro |
| Source: |
Frontiers in Cellular and Infection Microbiology, Vol 11 (2021) |
| Publisher Information: |
Frontiers Media S.A., 2021. |
| Publication Year: |
2021 |
| Collection: |
LCC:Microbiology |
| Subject Terms: |
Trypanosoma cruzi, dried blood spot (DBS), rapid diagnostic test (RDT), serological screening, community strategies, Microbiology, QR1-502 |
| More Details: |
BackgroundChagas disease is a public health problem not only in Latin America, but also in other regions, including Spain, due to migration movements. Conventional serological diagnosis requires an invasive sample (plasma or serum) and a well-equipped laboratory. To circumvent those limitations, blood samples dried on filter paper (DBS) or Rapid Diagnostic Test (RDT) could be a practical alternative to reference protocol for serological screening in epidemiological studies. We evaluated the usefulness of dried blood sampling and a rapid diagnostic test (Trypanosoma Detect™) for the detection of antibodies against T. cruzi for their use in community-based screening.Methodology/Principal FindingsA total of 162 stored paired whole-blood and serum samples from Latin American migrants and 25 negative-control blood samples were included. Diagnosis of chronic Chagas disease was performed in serum according to WHO algorithms. Blood samples were retrospectively collected as dried spots and then analyzed using two different serological techniques, enzyme-linked immunosorbent assay (ELISA) and electrochemiluminescence immunoassay (E-CLIA). Whole-blood samples were also used to evaluate a rapid diagnostic test based on immunochromatography. A better correlation with conventional serum was observed in dried blood elutes using E-CLIA than ELISA (97% vs. 77% sensitivity, respectively). Both assays reported 100% specificity. The median cut-off index values of E-CLIA for dried blood were significantly lower than those for serum (138.1 vs. 243.3, P |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2235-2988 |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fcimb.2021.736630/full; https://doaj.org/toc/2235-2988 |
| DOI: |
10.3389/fcimb.2021.736630 |
| Access URL: |
https://doaj.org/article/effffd2cf82448c98786201c579753cf |
| Accession Number: |
edsdoj.ffffd2cf82448c98786201c579753cf |
| Database: |
Directory of Open Access Journals |
