Tunable fractionation of nucleic acids

Bibliographic Details
Title:	Tunable fractionation of nucleic acids
Authors:	Md. Salimullah, Sachiko Kato, Mitsuyoshi Murata, Chika Kawazu, Charles Plessy, Piero Carninci
Source:	BioTechniques, Vol 47, Iss 6, Pp 1041-1043 (2009)
Publisher Information:	Taylor & Francis Group, 2009.
Publication Year:	2009
Collection:	LCC:Biology (General)
Subject Terms:	selective purification, CTAB, nucleic acids, size fractionation, Biology (General), QH301-705.5
More Details:	We developed a method for selective purification of DNA using the cationic detergent, cetyltrimethylammonium bromide (CTAB), accompanied with urea and controlled high-salt (NaCl) concentration. This method is effective for rapid separation of DNA fragments from artifacts such as PCR primer dimers or ligation adapters. The CTAB-associated purification completely removed the short PCR artifacts and primers, as well as enzymes and buffer, while recovering a sufficient quantity of amplicons for subsequent experiments such as preparation of libraries. This method could also be applied to the fractionation of nucleic acids generated by other types of reactions.
Document Type:	article
File Description:	electronic resource
Language:	English
ISSN:	1940-9818 0736-6205
Relation:	https://doaj.org/toc/0736-6205; https://doaj.org/toc/1940-9818
DOI:	10.2144/000113249
Access URL:	https://doaj.org/article/eff7a382559d4a1d862da3846312fcff
Accession Number:	edsdoj.ff7a382559d4a1d862da3846312fcff
Database:	Directory of Open Access Journals