Academic Journal
Rapid and unbiased enrichment of extracellular vesicles via a meticulously engineered peptide
| Title: | Rapid and unbiased enrichment of extracellular vesicles via a meticulously engineered peptide |
|---|---|
| Authors: | Le Wang, Zhou Gong, Ming Wang, Yi-Zhong Liang, Jing Zhao, Qi Xie, Xiao-Wei Wu, Qin-Ying Li, Cong Zhang, Li-Yun Ma, Si-Yang Zheng, Ming Jiang, Xu Yu, Li Xu |
| Source: | Bioactive Materials, Vol 43, Iss , Pp 292-304 (2025) |
| Publisher Information: | KeAi Communications Co., Ltd., 2025. |
| Publication Year: | 2025 |
| Collection: | LCC:Materials of engineering and construction. Mechanics of materials LCC:Biology (General) |
| Subject Terms: | Extracellular vesicles, Peptide, Protein assay, DNA mutation detection, Functionalized interface, Materials of engineering and construction. Mechanics of materials, TA401-492, Biology (General), QH301-705.5 |
| More Details: | Extracellular vesicles (EVs) have garnered significant attention in biomedical applications. However, the rapid, efficient, and unbiased separation of EVs from complex biological fluids remains a challenge due to their heterogeneity and low abundance in biofluids. Herein, we report a novel approach to reconfigure and modify an artificial insertion peptide for the unbiased and rapid isolation of EVs in 20 min with ∼80% recovery in neutral conditions. Moreover, the approach demonstrates exceptional anti-interference capability and achieves a high purity of EVs comparable to standard ultracentrifugation and other methods. Importantly, the isolated EVs could be directly applied for downstream protein and nucleic acid analyses, including proteomics analysis, exome sequencing analysis, as well as the detection of both epidermal growth factor receptor (EGFR) and V-Ki-ras2 Kirsten Rat Sarcoma Viral Oncogene Homologue (KRAS) gene mutation in clinical plasma samples. Our approach offers great possibilities for utilizing EVs in liquid biopsy, as well as in various other biomedical applications. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2452-199X |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2452199X24004183; https://doaj.org/toc/2452-199X |
| DOI: | 10.1016/j.bioactmat.2024.09.023 |
| Access URL: | https://doaj.org/article/ff22c6c76df54b7b9e0416c20c0b34ec |
| Accession Number: | edsdoj.ff22c6c76df54b7b9e0416c20c0b34ec |
| Database: | Directory of Open Access Journals |
| ISSN: | 2452199X |
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| DOI: | 10.1016/j.bioactmat.2024.09.023 |
| Published in: | Bioactive Materials |
| Language: | English |