Bibliographic Details
| Title: |
Characterization of T-complex polypeptide 1 (TCP-1) from the Chilo suppressalis HSP60 family and its expression in response to temperature stress |
| Authors: |
Tong-ying YU, Ming-xing LU, Ya-dong CUI |
| Source: |
Journal of Integrative Agriculture, Vol 17, Iss 5, Pp 1032-1039 (2018) |
| Publisher Information: |
Elsevier, 2018. |
| Publication Year: |
2018 |
| Collection: |
LCC:Agriculture (General) |
| Subject Terms: |
TCP-1, Chilo suppressalis, expression analysis, temperature stress, insect tissues, Agriculture (General), S1-972 |
| More Details: |
Many proteins require assistance from molecular chaperones at various stages to attain correctly folded states and functional conformations during protein synthesis. In this study, the gene encoding T-complex polypeptide 1 (TCP-1), which belongs to the heat shock protein 60 (HSP60) family, was isolated and characterized from the rice stem borer, Chilo suppressalis, by RACE and qPCR, respectively. The full-length cDNA of Tcp-1 was 2 144 bp and encoded a 1 635-bp ORF; the deduced translational product contained 545 amino acids with 5'- and 3'-UTRs and an isoelectric point of 5.29. Cluster analysis confirmed that the deduced amino acid sequence shared high identity (60–99%) with TCP-1 from other insects. To investigate Tcp-1 expression in response to abiotic stress, qPCR was used to analyze expression levels of Tcp-1 mRNA in C. suppressalis larvae exposed to temperatures ranging from –11 to 43°C. With respect to heat shock, Tcp-1 expression was higher than the control after a 2-h exposure to 30 and 36°C and declined at 39 and 43°C. Difference in Tcp-1 expression was observed at temperatures ranging from –11 to 27°C. qPCR analyses revealed that Tcp-1 expression was the highest in hindgut tissue as compared to heads, epidermis, fat body, foregut, midgut, and malpighian tubules. Our results indicated that Tcp-1 expression was differentially expressed in C. suppressalis tissues, and was impacted by temperature stress. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2095-3119 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2095311917617751; https://doaj.org/toc/2095-3119 |
| DOI: |
10.1016/S2095-3119(17)61775-1 |
| Access URL: |
https://doaj.org/article/efd3e55abe73442aa1d0ce6f27369e38 |
| Accession Number: |
edsdoj.fd3e55abe73442aa1d0ce6f27369e38 |
| Database: |
Directory of Open Access Journals |
