Academic Journal
Identification of new aptamer BC-3 targeting RPS7 from rapid screening for bladder carcinoma
| Title: | Identification of new aptamer BC-3 targeting RPS7 from rapid screening for bladder carcinoma |
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| Authors: | Yunyi Liu, Juan Li, Hailong Ou, Dan Qi, Bei Hu, Yuxi Xu, Jian Hu, Yi Xiong, Luling Xia, Jason H. Huang, Xiaoxiao Hu, Erxi Wu |
| Source: | Genes and Diseases, Vol 10, Iss 5, Pp 2137-2150 (2023) |
| Publisher Information: | KeAi Communications Co., Ltd., 2023. |
| Publication Year: | 2023 |
| Collection: | LCC:Medicine (General) LCC:Genetics |
| Subject Terms: | Bladder cancer, Cell-SELEX, Clathrin-mediated endocytosis, Intracellular colocalization, Ribosomal protein S7, X-aptamer selections, Medicine (General), R5-920, Genetics, QH426-470 |
| More Details: | Aptamers, short single DNA or RNA oligonucleotides, have shown immense application potential as molecular probes for the early diagnosis and therapy of cancer. However, conventional cell-SELEX technologies for aptamer discovery are time-consuming and laborious. Here we discovered a new aptamer BC-3 by using an improved rapid X-Aptamer selection process for human bladder carcinoma, for which there is no specific molecular probe yet. We show that BC-3 exhibited excellent affinity in bladder cancer cells but not normal cells. We demonstrate that BC-3 displayed high selectivity for tumor cells over their normal counterparts in vitro, in mice, and in patient tumor tissue specimens. Further endocytosis pathway analysis revealed that BC-3 internalized into bladder cancer cells via clathrin-mediated endocytosis. Importantly, we identified ribosomal protein S7 (RPS7) as the binding target of BC-3 via an integrated methodology (mass spectrometry, colocalization assay, and immunoblotting). Together, we report that a novel aptamer BC-3 is discovered for bladder cancer and its properties in the disease are unearthed. Our findings will facilitate the discovery of novel diagnostic and therapeutic strategies for bladder cancer. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2352-3042 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2352304222001982; https://doaj.org/toc/2352-3042 |
| DOI: | 10.1016/j.gendis.2022.07.002 |
| Access URL: | https://doaj.org/article/fc691fe7304747e591723a313630dae2 |
| Accession Number: | edsdoj.fc691fe7304747e591723a313630dae2 |
| Database: | Directory of Open Access Journals |
| ISSN: | 23523042 |
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| DOI: | 10.1016/j.gendis.2022.07.002 |
| Published in: | Genes and Diseases |
| Language: | English |