Bibliographic Details
| Title: |
Effect of 6-hydroxydopamine blockade of sympathetic nerves on systemic inflammation in an imiquimod-induced psoriasis-like mouse model |
| Authors: |
Siqi LIU, Yurong LUO, Yu PENG, Yingping XU, Yunsheng LIANG |
| Source: |
Pifu-xingbing zhenliaoxue zazhi, Vol 29, Iss 3, Pp 212-219 (2022) |
| Publisher Information: |
editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology, 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Dermatology |
| Subject Terms: |
psoriasis, imiquimod, systemic inflammation, 6-ohda, Dermatology, RL1-803 |
| More Details: |
Objective To explore the effect of 6-hydroxydopamine blockade of sympathetic nerves on systemic inflammation in an imiquimod(IMQ)-induced psoriasis-like mouse model. Methods Twenty-four SPF C57BL/6J mice were randomly divided into psoriasis-like dermatitis model group (model group), Candida albicans infected group, blank control group, psoriasis-like dermatitis model with vehicle treatment group (vehicle model group), 6-hydroxydopamine (6-OHDA) treated group, vehicle control group, with 4 mice in each group. The changes of lesion in each group were observed every day. Local inflammation in the dorsal and ear lesions of mice was detected by HE staining, q-PCR and Western blotting of interleukin 17A(IL-17A). Spleens of mice were taken to detect inflammatory cells by flow cytometry, and the level of interleukin 6(IL-6) and interleukin 1β(IL-1β) were detected by q-PCR and Western blotting for the systemic inflammation. Results Epidermal thickening, hypokeratosis, basal keratinocyte proliferation, inflammatory cell infiltration and IL-17A increasing were observed on the dorsal lesions of mice in the Candida albicans infection group and the psoriasis-like model group. Compared with the Candida albicans infected group, there were a large number of skin scale production and spleen enlargement, and splenic inflammatory cells increased, the level of IL-6, IL-1β increased in the psoriasis-like model group. Compared with the solvent model group, the spleen in the 6-OHDA treated group was smaller, the number of macrophages derived from neutrophils and monocytes in the spleen was significantly decrease, and the levels of IL-6 and IL-1β in the spleen were significantly lower. Conclusions IMQ-induced psoriasis-like mouse model lead to systemic inflammation, which can be caused by excessive sympathetic activation. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
Chinese |
| ISSN: |
1674-8468 |
| Relation: |
http://pfxbzlx.gdvdc.com/CN/abstract/abstract2074.shtml; https://doaj.org/toc/1674-8468 |
| DOI: |
10.3969/j.issn.1674-8468.2022.03.004 |
| Access URL: |
https://doaj.org/article/fbfc663d36fd423eb6f12f2ee9a4988d |
| Accession Number: |
edsdoj.fbfc663d36fd423eb6f12f2ee9a4988d |
| Database: |
Directory of Open Access Journals |
