Bibliographic Details
| Title: |
Punicalagin Inhibits African Swine Fever Virus Replication by Targeting Early Viral Stages and Modulating Inflammatory Pathways |
| Authors: |
Renhao Geng, Dan Yin, Yingnan Liu, Hui Lv, Xiaoyu Zhou, Chunhui Bao, Lang Gong, Hongxia Shao, Kun Qian, Hongjun Chen, Aijian Qin |
| Source: |
Veterinary Sciences, Vol 11, Iss 9, p 440 (2024) |
| Publisher Information: |
MDPI AG, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Veterinary medicine |
| Subject Terms: |
African swine fever virus, library screen, punicalagin, replication stages, NF-κB/STAT3/NLRP3, Veterinary medicine, SF600-1100 |
| More Details: |
African swine fever (ASF), caused by the African swine fever virus (ASFV), has resulted in significant losses in the global pig industry. Considering the absence of effective vaccines, developing drugs against ASFV may be a crucial strategy for its prevention and control in the future. In this study, punicalagin, a polyphenolic substance extracted from pomegranate peel, was found to significantly inhibit ASFV replication in MA-104, PK-15, WSL, and 3D4/21 cells by screening an antiviral compound library containing 536 compounds. Time-of-addition studies demonstrated that punicalagin acted on early viral replication stages, impinging on viral attachment and internalization. Meanwhile, punicalagin could directly inactivate the virus according to virucidal assay. RT-qPCR and Western blot results indicated that punicalagin modulated the NF-κB/STAT3/NLRP3 inflammasome signaling pathway and reduced the levels of inflammatory mediators induced by ASFV. In conclusion, this study reveals the anti-ASFV activity of punicalagin and the mechanism of action, which may have great potential for developing effective drugs against ASFV. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2306-7381 |
| Relation: |
https://www.mdpi.com/2306-7381/11/9/440; https://doaj.org/toc/2306-7381 |
| DOI: |
10.3390/vetsci11090440 |
| Access URL: |
https://doaj.org/article/cfa0e05ded0840d9820baef3b4d1cb4c |
| Accession Number: |
edsdoj.fa0e05ded0840d9820baef3b4d1cb4c |
| Database: |
Directory of Open Access Journals |
