Academic Journal
miRNA-541-5p regulates myocardial ischemia–reperfusion injury by targeting ferroptosis
| Title: | miRNA-541-5p regulates myocardial ischemia–reperfusion injury by targeting ferroptosis |
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| Authors: | ZhiYu Zhao, BoXia Li, DianWei Cheng, YuFang Leng |
| Source: | Journal of Cardiothoracic Surgery, Vol 20, Iss 1, Pp 1-24 (2025) |
| Publisher Information: | BMC, 2025. |
| Publication Year: | 2025 |
| Collection: | LCC:Surgery LCC:Anesthesiology |
| Subject Terms: | Myocardial ischemia–reperfusion injury, Ferroptosis, MiRNA-541-5p, MiRNA sequencing, Surgery, RD1-811, Anesthesiology, RD78.3-87.3 |
| More Details: | Abstract Background This article aims to use high-throughput sequencing to identify miRNAs associated with ferroptosis in myocardial ischemia–reperfusion injury, select a target miRNA, and investigate its role in H9C2 cells hypoxia-reoxygenation injury. Methods SD rats and H9C2 cells were used as subjects. ELISA kits quantified MDA, SOD, GSH, LDH, and ferritin levels. TTC staining evaluated infarction size. HE staining observed histopathological changes. DCFH-DA fluorescent probe detected ROS. CCK-8 kit measured cell viability. HiSeq 2000 sequencing performed differential expression analysis of miRNAs. qRT-PCR and Western blots assessed the expression levels of GPX-4, ACSL-4, HO-1, TFR-1 and TFR-2. SPSS 21.0 software conducted statistical analysis. Results Myocardial ischemia–reperfusion injury resulted in decreased levels of SOD and GSH, increased levels of LDH and MDA, up-regulation of ferritin, ACSL-4, HO-1, and TFR-2, down-regulation of GPX-4, increased tissue damage, and accumulation of ROS. However, DFO treatment reversed these changes. Subsequently, the target gene miRNA-541-5p was obtained by miRNA sequencing screening, and further validation revealed that miRNA-541-5p expression was increased in the myocardial tissues of rats in the I/R injury model group compared with those of rats in the NC group, P |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1749-8090 |
| Relation: | https://doaj.org/toc/1749-8090 |
| DOI: | 10.1186/s13019-024-03260-2 |
| Access URL: | https://doaj.org/article/f9f4afad61604ffa8930fa93f064f9ed |
| Accession Number: | edsdoj.f9f4afad61604ffa8930fa93f064f9ed |
| Database: | Directory of Open Access Journals |
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| ISSN: | 17498090 |
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| DOI: | 10.1186/s13019-024-03260-2 |
| Published in: | Journal of Cardiothoracic Surgery |
| Language: | English |