Academic Journal
A systematic morphology study on the effect of high glucose on intervertebral disc endplate degeneration in mice
| Title: | A systematic morphology study on the effect of high glucose on intervertebral disc endplate degeneration in mice |
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| Authors: | Huilin Quan, Xiaoshuang Zuo, Yu Huan, Xuankang Wang, Zhou Yao, Chunmei Wang, Fang Ren, Hong Wang, Hongyan Qin, Xueyu Hu |
| Source: | Heliyon, Vol 9, Iss 2, Pp e13295- (2023) |
| Publisher Information: | Elsevier, 2023. |
| Publication Year: | 2023 |
| Collection: | LCC:Science (General) LCC:Social sciences (General) |
| Subject Terms: | Diabetes, Endplate, Vascular invasion, Monocyte/macrophage infiltration, Inflammation, Science (General), Q1-390, Social sciences (General), H1-99 |
| More Details: | To explore the relationship between diabetes and intervertebral disc degeneration in mice and the associated underlying mechanism. Four-week-old male Kunming mice were used to model diabetes using a high-fat diet combined with streptozotocin injection. After 6 months, morphological and pathological changes in L4-L6 intervertebral discs were detected by magnetic resonance imaging, micro-CT and histological staining. Immunostaining of CD31, F4/80 and CD16/32 receptors was used to detect vascular invasion and inflammatory infiltration in endplates; the exact changes were then explored by the transmission electron microscopy. The nucleus pulposus of the control and the diabetic group had a clear boundary and regular shape without collapse, while endplate calcification and chondrocyte abnormality in the diabetic group were more obvious. Immunofluorescence confirmed that compared to control, expression levels of CD31 (vascular endothelial marker) and F4/80 (monocyte/macrophage marker) in the diabetic group were significantly increased (P |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2405-8440 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2405844023005029; https://doaj.org/toc/2405-8440 |
| DOI: | 10.1016/j.heliyon.2023.e13295 |
| Access URL: | https://doaj.org/article/f8fffa4f824a4d5d9f2cbf07564f5715 |
| Accession Number: | edsdoj.f8fffa4f824a4d5d9f2cbf07564f5715 |
| Database: | Directory of Open Access Journals |
| ISSN: | 24058440 |
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| DOI: | 10.1016/j.heliyon.2023.e13295 |
| Published in: | Heliyon |
| Language: | English |