Academic Journal
Strategic Combination of Isocratic and Gradient Elution for Simultaneous Separation of Polar Compounds in Traditional Chinese Medicines by HPLC
| Title: | Strategic Combination of Isocratic and Gradient Elution for Simultaneous Separation of Polar Compounds in Traditional Chinese Medicines by HPLC |
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| Authors: | Mei-Tuo Zhang, Xiao-Xue Ye, Wei Lan, Yan-Ling Yang, Tsunghsueh Wu, Yu-Sang Li, He-Bin Tang |
| Source: | Journal of Analytical Methods in Chemistry, Vol 2018 (2018) |
| Publisher Information: | Hindawi Limited, 2018. |
| Publication Year: | 2018 |
| Collection: | LCC:Analytical chemistry |
| Subject Terms: | Analytical chemistry, QD71-142 |
| More Details: | A simple high-performance liquid chromatography (HPLC) method for the simultaneous separation of the highly polar and weakly polar components of traditional Chinese medicines was developed via a strategic combination of isocratic and gradient elution methods. Liu-Shen-Wan and Liu-Wei-Di-Huang-Wan were used as representative examples of traditional Chinese medicines. This is the first time that 6 components of varying degrees of polarity in Liu-Shen-Wan had been successfully resolved in a single chromatographic run using an ultraviolet-visible detector with a fixed wavelength of 296 nm. In contrast to conventional gradient separation methods, this novel method offered a viable route for separation of the highly and weakly polar fractions simultaneously, thus greatly reducing the time and cost of analysis. This method therefore provides a more efficient way to determine the polar components present in traditional Chinese medicines. It would find potential application in drug screening, drug authentication, and product quality control. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2090-8865 2090-8873 |
| Relation: | https://doaj.org/toc/2090-8865; https://doaj.org/toc/2090-8873 |
| DOI: | 10.1155/2018/7569283 |
| Access URL: | https://doaj.org/article/af8ad00457144359934d4b2cbc9d3995 |
| Accession Number: | edsdoj.f8ad00457144359934d4b2cbc9d3995 |
| Database: | Directory of Open Access Journals |
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| ISSN: | 20908865 20908873 |
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| DOI: | 10.1155/2018/7569283 |
| Published in: | Journal of Analytical Methods in Chemistry |
| Language: | English |