Bibliographic Details
| Title: |
A role for polyploidy in the tumorigenicity of Pim-1-expressing human prostate and mammary epithelial cells. |
| Authors: |
Meejeon Roh, Omar E Franco, Simon W Hayward, Riet van der Meer, Sarki A Abdulkadir |
| Source: |
PLoS ONE, Vol 3, Iss 7, p e2572 (2008) |
| Publisher Information: |
Public Library of Science (PLoS), 2008. |
| Publication Year: |
2008 |
| Collection: |
LCC:Medicine
LCC:Science |
| Subject Terms: |
Medicine, Science |
| More Details: |
BackgroundPolyploidy is a prominent feature of many human cancers, and it has long been hypothesized that polyploidy may contribute to tumorigenesis by promoting genomic instability. In this study, we investigated whether polyploidy per se induced by a relevant oncogene can promote genomic instability and tumorigenicity in human epithelial cells.Principal findingsWhen the oncogenic serine-threonine kinase Pim-1 is overexpressed in immortalized, non-tumorigenic human prostate and mammary epithelial cells, these cells gradually converted to polyploidy and became tumorigenic. To assess the contribution of polyploidy to tumorigenicity, we obtained sorted, matched populations of diploid and polyploid cells expressing equivalent levels of the Pim-1 protein. Spectral karyotyping revealed evidence of emerging numerical and structural chromosomal abnormalities in polyploid cells, supporting the proposition that polyploidy promotes chromosomal instability. Polyploid cells displayed an intact p53/p21 pathway, indicating that the viability of polyploid cells in this system is not dependent on the inactivation of the p53 signaling pathway. Remarkably, only the sorted polyploid cells were tumorigenic in vitro and in vivo.ConclusionsOur results support the notion that polyploidy can promote chromosomal instability and the initiation of tumorigenesis in human epithelial cells. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1932-6203 |
| Relation: |
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18596907/pdf/?tool=EBI; https://doaj.org/toc/1932-6203 |
| DOI: |
10.1371/journal.pone.0002572 |
| Access URL: |
https://doaj.org/article/f7c3d302cc6643d9942ab3d9f8a02b8d |
| Accession Number: |
edsdoj.f7c3d302cc6643d9942ab3d9f8a02b8d |
| Database: |
Directory of Open Access Journals |
