Bibliographic Details
| Title: |
Associations Between a Polymorphism in the Rat 5-HT1A Receptor Gene Promoter Region (rs198585630) and Cognitive Alterations Induced by Microwave Exposure |
| Authors: |
Haijuan Li, Yu Gao, Yong Zou, Simo Qiao, Weijia Zhi, Lizhen Ma, Xinping Xu, Xuelong Zhao, Junhua Zhang, Lifeng Wang, Xiangjun Hu |
| Source: |
Frontiers in Public Health, Vol 10 (2022) |
| Publisher Information: |
Frontiers Media S.A., 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Public aspects of medicine |
| Subject Terms: |
microwave, 5-HT1A receptor, SNP, cognition, association, Public aspects of medicine, RA1-1270 |
| More Details: |
The nervous system is a sensitive target of electromagnetic radiation (EMR). Chronic microwave exposure can induce cognitive deficits, and 5-HT system is involved in this effect. Genetic polymorphisms lead to individual differences. In this study, we evaluated whether the single-nucleotide polymorphism (SNP) rs198585630 of 5-HT1A receptor is associated with cognitive alterations in rats after microwave exposure with a frequency of 2.856 GHz and an average power density of 30 mW/cm2. Rats were exposed to microwaves for 6 min three times a week for up to 6 weeks. PC12 cells and 293T cells were exposed to microwaves for 5 min up to 3 times at 2 intervals of 5 min. Transcriptional activity of 5-HT1A receptor promoter containing rs198585630 C/T allele was determined in vitro. Electroencephalograms (EEGs), spatial learning and memory, and mRNA and protein expression of 5-HT1A receptor were evaluated in vivo. We demonstrated that transcriptional activity of 5-HT1A receptor promoter containing rs198585630 C allele was higher than that of 5-HT1A receptor promoter containing T allele. The transcriptional activity of 5-HT1A receptor promoter was stimulated by 30 mW/cm2 microwave exposure, and rs198585630 C allele was more sensitive to microwave exposure, as it showed stronger transcriptional activation. Rats carrying rs198585630 C allele exhibited increased mRNA and protein expression of 5-HT1A receptor and were more susceptible to 30 mW/cm2 microwave exposure, showing cognitive deficits and inhibition of brain electrical activity. These findings suggest SNP rs198585630 of the 5-HT1A receptor is an important target for further research exploring the mechanisms of hypersensitivity to microwave exposure. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2296-2565 |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fpubh.2022.802386/full; https://doaj.org/toc/2296-2565 |
| DOI: |
10.3389/fpubh.2022.802386 |
| Access URL: |
https://doaj.org/article/df6cd5246fd2462397875d0f7703d857 |
| Accession Number: |
edsdoj.f6cd5246fd2462397875d0f7703d857 |
| Database: |
Directory of Open Access Journals |
