Test and Evaluation of the Made-in-China Quick TargSeq Fully Integrated Automated On-site Rapid DNA Detection Instrument

Bibliographic Details
Title: Test and Evaluation of the Made-in-China Quick TargSeq Fully Integrated Automated On-site Rapid DNA Detection Instrument
Authors: HAN Jun-ping, WANG Ying-xi, LIU Chao, LIU Feng, GUO Jin-rong, ZHU Wei, TANG Guang-feng, LI Cai-xia, WANG Xin, JIAO Zhang-ping
Source: Fayixue Zazhi, Vol 40, Iss 5, Pp 461-467 (2024)
Publisher Information: Editorial Office of Journal of Forensic Medicine, 2024.
Publication Year: 2024
Collection: LCC:Medicine
Subject Terms: forensic genetics, forensic dna typing, rapid dna analysis, short tandem repeat, ancestry inference, Medicine
More Details: ObjectiveTo verify the stability and accuracy of the made-in-China Quick TargSeq Fully Integrated Automated On-site Rapid DNA Detection Instrument (hereinafter referred to as Quick TargSeq DNA detection instrument) and evaluate its forensic application prospects.MethodsA total of 74 blind samples provided by the expert group of Criminal Investigation Bureau of the Ministry of Public Security (4 individuals, 20 samples) and five participating institutions (54 samples) were used to test on 5 Quick TargSeq DNA detection instruments and the accompanying microfluidic chip cartridges. The results were compared with the known genotypes to calculate the samples’ detection rate and genotyping accuracy.ResultsThe results of reproducibility obtained from 20 samples provided by the expert group on the 5 Quick TargSeq DNA detection instruments were all consistent with the known genotypes. The detection rate of the 74 samples was 100%, and the genotyping accuracy was 97.33%.ConclusionThe Quick TargSeq DNA detection instrument has good stability and high genotyping accuracy.
Document Type: article
File Description: electronic resource
Language: Chinese
ISSN: 1004-5619
Relation: http://www.fyxzz.cn/article/2024/1004-5619/1004-5619-2024-40-5-461.shtml; https://doaj.org/toc/1004-5619
DOI: 10.12116/j.issn.1004-5619.2023.530601
Access URL: https://doaj.org/article/df62edb3e6b3458da7ece89ed5cdd865
Accession Number: edsdoj.f62edb3e6b3458da7ece89ed5cdd865
Database: Directory of Open Access Journals
More Details
ISSN:10045619
DOI:10.12116/j.issn.1004-5619.2023.530601
Published in:Fayixue Zazhi
Language:Chinese