Academic Journal
View in EDS

Exogenous Sodium Nitroprusside Affects the Redox System of Wheat Roots Differentially Regulating the Activity of Antioxidant Enzymes under Short-Time Osmotic Stress

Bibliographic Details
Title: Exogenous Sodium Nitroprusside Affects the Redox System of Wheat Roots Differentially Regulating the Activity of Antioxidant Enzymes under Short-Time Osmotic Stress
Authors: Alsu Lubyanova, Chulpan Allagulova
Source: Plants, Vol 13, Iss 14, p 1895 (2024)
Publisher Information: MDPI AG, 2024.
Publication Year: 2024
Collection: LCC:Botany
Subject Terms: catalase, electrolyte leakage, hydrogen peroxide, malondialdehyde, ascorbate peroxidase, polyethylene glycol, Botany, QK1-989
More Details: Nitric oxide (NO) is a multifunctional signalling molecule involved in the regulation of plant ontogenesis and adaptation to different adverse environmental factors, in particular to osmotic stress. Understanding NO-induced plant protection is important for the improvement of plant stress tolerance and crop productivity under global climate changes. The root system is crucial for plant survival in a changeable environment. Damages that it experiences under water deficit conditions during the initial developmental periods seriously affect the viability of the plants. This work was devoted to the comparative analysis of the pretreatment of wheat seedlings through the root system with NO donor sodium nitroprusside (SNP) for 24 h on various parameters of redox homeostasis under exposure to osmotic stress (PEG 6000, 12%) over 0.5–24 h. The active and exhausted solutions of SNP, termed as (SNP/+NO) and (SNP/−NO), respectively, were used in this work at a concentration of 2 × 10−4 M. Using biochemistry and light microscopy methods, it has been revealed that osmotic stress caused oxidative damages and the disruption of membrane cell structures in wheat roots. PEG exposure increased the production of superoxide (O2•−), hydrogen peroxide (H2O2), malondialdehyde (MDA), and the levels of electrolyte leakage (EL) and lipid peroxidation (LPO). Stress treatment enhanced the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), the excretion of proline, and the rate of cell death and inhibited their division. Pretreatment with (SNP/+NO) decreased PEG-induced root damages by differently regulating the antioxidant enzymes under stress conditions. Thus, (SNP/+NO) pretreatment led to SOD, APX, and CAT inhibition during the first 4 h of stress and stimulated their activity after 24 h of PEG exposure when compared to SNP-untreated or (SNP/−NO)-pretreated and stress-subjected plants. Osmotic stress triggered the intense excretion of proline by roots into the external medium. Pretreatment with (SNP/+NO) in contrast with (SNP/−NO) additionally increased stress-induced proline excretion. Our results indicate that NO is able to mitigate the destructive effects of osmotic stress on the roots of wheat seedlings. However, the mechanisms of NO protective action may be different at certain periods of stress exposure.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2223-7747
Relation: https://www.mdpi.com/2223-7747/13/14/1895; https://doaj.org/toc/2223-7747
DOI: 10.3390/plants13141895
Access URL: https://doaj.org/article/f5e68a54e07d48e69ae9dafc8536696d
Accession Number: edsdoj.f5e68a54e07d48e69ae9dafc8536696d
Database: Directory of Open Access Journals
FullText Text:
  Availability: 0
CustomLinks:
  – Url: https://resolver.ebsco.com/c/xy5jbn/result?sid=EBSCO:edsdoj&genre=article&issn=22237747&ISBN=&volume=13&issue=14&date=20240701&spage=1895&pages=1895-1895&title=Plants&atitle=Exogenous%20Sodium%20Nitroprusside%20Affects%20the%20Redox%20System%20of%20Wheat%20Roots%20Differentially%20Regulating%20the%20Activity%20of%20Antioxidant%20Enzymes%20under%20Short-Time%20Osmotic%20Stress&aulast=Alsu%20Lubyanova&id=DOI:10.3390/plants13141895
    Name: Full Text Finder (for New FTF UI) (s8985755)
    Category: fullText
    Text: Find It @ SCU Libraries
    MouseOverText: Find It @ SCU Libraries
  – Url: https://doaj.org/article/f5e68a54e07d48e69ae9dafc8536696d
    Name: EDS - DOAJ (s8985755)
    Category: fullText
    Text: View record from DOAJ
    MouseOverText: View record from DOAJ
Header DbId: edsdoj
DbLabel: Directory of Open Access Journals
An: edsdoj.f5e68a54e07d48e69ae9dafc8536696d
RelevancyScore: 997
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 997.228698730469
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Exogenous Sodium Nitroprusside Affects the Redox System of Wheat Roots Differentially Regulating the Activity of Antioxidant Enzymes under Short-Time Osmotic Stress
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Alsu+Lubyanova%22">Alsu Lubyanova</searchLink><br /><searchLink fieldCode="AR" term="%22Chulpan+Allagulova%22">Chulpan Allagulova</searchLink>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: Plants, Vol 13, Iss 14, p 1895 (2024)
– Name: Publisher
  Label: Publisher Information
  Group: PubInfo
  Data: MDPI AG, 2024.
– Name: DatePubCY
  Label: Publication Year
  Group: Date
  Data: 2024
– Name: Subset
  Label: Collection
  Group: HoldingsInfo
  Data: LCC:Botany
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22catalase%22">catalase</searchLink><br /><searchLink fieldCode="DE" term="%22electrolyte+leakage%22">electrolyte leakage</searchLink><br /><searchLink fieldCode="DE" term="%22hydrogen+peroxide%22">hydrogen peroxide</searchLink><br /><searchLink fieldCode="DE" term="%22malondialdehyde%22">malondialdehyde</searchLink><br /><searchLink fieldCode="DE" term="%22ascorbate+peroxidase%22">ascorbate peroxidase</searchLink><br /><searchLink fieldCode="DE" term="%22polyethylene+glycol%22">polyethylene glycol</searchLink><br /><searchLink fieldCode="DE" term="%22Botany%22">Botany</searchLink><br /><searchLink fieldCode="DE" term="%22QK1-989%22">QK1-989</searchLink>
– Name: Abstract
  Label: Description
  Group: Ab
  Data: Nitric oxide (NO) is a multifunctional signalling molecule involved in the regulation of plant ontogenesis and adaptation to different adverse environmental factors, in particular to osmotic stress. Understanding NO-induced plant protection is important for the improvement of plant stress tolerance and crop productivity under global climate changes. The root system is crucial for plant survival in a changeable environment. Damages that it experiences under water deficit conditions during the initial developmental periods seriously affect the viability of the plants. This work was devoted to the comparative analysis of the pretreatment of wheat seedlings through the root system with NO donor sodium nitroprusside (SNP) for 24 h on various parameters of redox homeostasis under exposure to osmotic stress (PEG 6000, 12%) over 0.5–24 h. The active and exhausted solutions of SNP, termed as (SNP/+NO) and (SNP/−NO), respectively, were used in this work at a concentration of 2 × 10−4 M. Using biochemistry and light microscopy methods, it has been revealed that osmotic stress caused oxidative damages and the disruption of membrane cell structures in wheat roots. PEG exposure increased the production of superoxide (O2•−), hydrogen peroxide (H2O2), malondialdehyde (MDA), and the levels of electrolyte leakage (EL) and lipid peroxidation (LPO). Stress treatment enhanced the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), the excretion of proline, and the rate of cell death and inhibited their division. Pretreatment with (SNP/+NO) decreased PEG-induced root damages by differently regulating the antioxidant enzymes under stress conditions. Thus, (SNP/+NO) pretreatment led to SOD, APX, and CAT inhibition during the first 4 h of stress and stimulated their activity after 24 h of PEG exposure when compared to SNP-untreated or (SNP/−NO)-pretreated and stress-subjected plants. Osmotic stress triggered the intense excretion of proline by roots into the external medium. Pretreatment with (SNP/+NO) in contrast with (SNP/−NO) additionally increased stress-induced proline excretion. Our results indicate that NO is able to mitigate the destructive effects of osmotic stress on the roots of wheat seedlings. However, the mechanisms of NO protective action may be different at certain periods of stress exposure.
– Name: TypeDocument
  Label: Document Type
  Group: TypDoc
  Data: article
– Name: Format
  Label: File Description
  Group: SrcInfo
  Data: electronic resource
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: ISSN
  Label: ISSN
  Group: ISSN
  Data: 2223-7747
– Name: NoteTitleSource
  Label: Relation
  Group: SrcInfo
  Data: https://www.mdpi.com/2223-7747/13/14/1895; https://doaj.org/toc/2223-7747
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.3390/plants13141895
– Name: URL
  Label: Access URL
  Group: URL
  Data: <link linkTarget="URL" linkTerm="https://doaj.org/article/f5e68a54e07d48e69ae9dafc8536696d" linkWindow="_blank">https://doaj.org/article/f5e68a54e07d48e69ae9dafc8536696d</link>
– Name: AN
  Label: Accession Number
  Group: ID
  Data: edsdoj.f5e68a54e07d48e69ae9dafc8536696d
PLink https://login.libproxy.scu.edu/login?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsdoj&AN=edsdoj.f5e68a54e07d48e69ae9dafc8536696d
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.3390/plants13141895
    Languages:
      – Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 1
        StartPage: 1895
    Subjects:
      – SubjectFull: catalase
        Type: general
      – SubjectFull: electrolyte leakage
        Type: general
      – SubjectFull: hydrogen peroxide
        Type: general
      – SubjectFull: malondialdehyde
        Type: general
      – SubjectFull: ascorbate peroxidase
        Type: general
      – SubjectFull: polyethylene glycol
        Type: general
      – SubjectFull: Botany
        Type: general
      – SubjectFull: QK1-989
        Type: general
    Titles:
      – TitleFull: Exogenous Sodium Nitroprusside Affects the Redox System of Wheat Roots Differentially Regulating the Activity of Antioxidant Enzymes under Short-Time Osmotic Stress
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Alsu Lubyanova
      – PersonEntity:
          Name:
            NameFull: Chulpan Allagulova
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 07
              Type: published
              Y: 2024
          Identifiers:
            – Type: issn-print
              Value: 22237747
          Numbering:
            – Type: volume
              Value: 13
            – Type: issue
              Value: 14
          Titles:
            – TitleFull: Plants
              Type: main
ResultId 1