Blister Fluid Induces MMP-9-Associated M2-Type Macrophages in Bullous Pemphigoid

Bibliographic Details
Title: Blister Fluid Induces MMP-9-Associated M2-Type Macrophages in Bullous Pemphigoid
Authors: Meriem Riani, Céline Muller, Camille Bour, Philippe Bernard, Frank Antonicelli, Sébastien Le Jan
Source: Frontiers in Immunology, Vol 10 (2019)
Publisher Information: Frontiers Media S.A., 2019.
Publication Year: 2019
Collection: LCC:Immunologic diseases. Allergy
Subject Terms: bullous pemphigoid, autoimmunity, inflammation, macrophage polarization, MMP-9, Immunologic diseases. Allergy, RC581-607
More Details: Bullous pemphigoid (BP) is a cutaneous autoimmune disease, characterized by an inflammatory cascade leading to blister formation. Although macrophages were shown to participate in BP pathophysiology, their role in the blister formation process still needs to be investigated. We here addressed the influence of serum and blister fluid (BF) from patients with BP on the polarization status of macrophages with regards to the metalloproteinase-9 (MMP-9) expression. We demonstrated that several markers related to the alternatively activated macrophage phenotype (M2) including IL-10, TARC, arginase, TNFα, and IL-1RA were meaningfully increased in BF of patients with BP. We further showed that BF, but not serum from patients with BP, significantly induced the expression of CD163, CD206, and IL-10 in BP monocyte-derived macrophages (MDMs). Notably IL-10 was the only cytokine to be correlated to the reference clinical score, BP disease activity index (BPDAI), especially to the inflammatory BPDAI subscore evaluating urticarial and erythematous skin lesions (r = 0.57, p = 0.0004). We also found elevated levels of MMP-9 to M2-type macrophages ex vivo and highlighted the presence of CD163+ MMP-9+ macrophages histologically, at skin lesional site. Finally, we showed that methylprednisolone reduced MMP-9 levels in MDMs without modifying the other M2 markers. All together these results strongly support the presence of M2-phenotype macrophages with pro-inflammatory properties susceptible to favor blister formation in BP.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 1664-3224
Relation: https://www.frontiersin.org/article/10.3389/fimmu.2019.01858/full; https://doaj.org/toc/1664-3224
DOI: 10.3389/fimmu.2019.01858
Access URL: https://doaj.org/article/af5c7e0f47274cda8d09c357f64f14d9
Accession Number: edsdoj.f5c7e0f47274cda8d09c357f64f14d9
Database: Directory of Open Access Journals
More Details
ISSN:16643224
DOI:10.3389/fimmu.2019.01858
Published in:Frontiers in Immunology
Language:English