Osthole attenuated cytotoxicity induced by 6-OHDA in SH-SY5Y cells through inhibition of JAK/STAT and MAPK pathways
| Title: | Osthole attenuated cytotoxicity induced by 6-OHDA in SH-SY5Y cells through inhibition of JAK/STAT and MAPK pathways |
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| Authors: | Samira Barangi, Parisa Hosseinizadeh, Gholamreza Karimi, Zahra Tayarani‑Najaran, Soghra Mehri |
| Source: | Iranian Journal of Basic Medical Sciences, Vol 26, Iss 8, Pp 953-959 (2023) |
| Publisher Information: | Mashhad University of Medical Sciences, 2023. |
| Publication Year: | 2023 |
| Collection: | LCC:Medicine |
| Subject Terms: | 6-ohda, apoptosis, jak kinases, mitogen-activated protein-kinase, osthole, stat3, Medicine |
| More Details: | Objective(s): Natural coumarin called osthole is regarded as a medicinal herb with widespread applications in Traditional Chinese Medicine. It has various pharmacological properties, including antioxidant, anti-inflammatory, and anti-apoptotic effects. In some neurodegenerative diseases, osthole also shows neuroprotective properties. In this study, we explored how osthole protects human neuroblastoma SH-SY5Y cells from the cytotoxicity of 6-hydroxydopamine (6-OHDA). Materials and Methods: Using the MTT assay and DCFH-DA methods, respectively, the viability of the cells and the quantity of intracellular reactive oxygen species (ROS) were evaluated. Signal Transducers and Activators of Transcription (STAT), Janus Kinase (JAK), extracellular signal-regulated kinase 1/2 (ERK1/2), c-Jun N-terminal kinase (JNK), and caspase-3 activation levels were examined using western blotting.Results: In SH-SY5Y cells, the results showed that a 24-hour exposure to 6-OHDA (200 µM) lowered cell viability but markedly elevated ROS, p-JAK/JAK, p-STAT/STAT, p-ERK/ERK, p-JNK/JNK ratio, and caspase-3 levels. Interestingly, osthole (100 µM) pretreatment of cells for 24 hr prevented 6-OHDA-induced cytotoxicity by undoing all effects of 6-OHDA. Conclusion: In summary, our data showed that osthole protects SH-SY5Y cells against 6-OHDA-induced cytotoxicity by inhibiting ROS generation and reducing the activity of the JAK/STAT, MAPK, and apoptotic pathways. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2008-3866 2008-3874 |
| Relation: | https://ijbms.mums.ac.ir/article_22478_513101a8c99bf55f52e50d913a35ee5b.pdf; https://doaj.org/toc/2008-3866; https://doaj.org/toc/2008-3874 |
| DOI: | 10.22038/ijbms.2023.68292.14905 |
| Access URL: | https://doaj.org/article/f5167d3c974a4e11933d66e1e50b2bd8 |
| Accession Number: | edsdoj.f5167d3c974a4e11933d66e1e50b2bd8 |
| Database: | Directory of Open Access Journals |
| ISSN: | 20083866 20083874 |
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| DOI: | 10.22038/ijbms.2023.68292.14905 |
| Published in: | Iranian Journal of Basic Medical Sciences |
| Language: | English |