Academic Journal
An Isoform of the Oncogenic Splice Variant AIMP2-DX2 Detected by a Novel Monoclonal Antibody
| Title: | An Isoform of the Oncogenic Splice Variant AIMP2-DX2 Detected by a Novel Monoclonal Antibody |
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| Authors: | Dae Gyu Kim, Thi Thu Ha Nguyen, Nam Hoon Kwon, Junsik Sung, Semi Lim, Eun-Joo Kang, Jihye Lee, Woo Young Seo, Arum Kim, Yoon Soo Chang, Hyunbo Shim, Sunghoon Kim |
| Source: | Biomolecules, Vol 10, Iss 6, p 820 (2020) |
| Publisher Information: | MDPI AG, 2020. |
| Publication Year: | 2020 |
| Collection: | LCC:Microbiology |
| Subject Terms: | antibody, splice variant, AIMP2-DX2, phage display, diagnostic marker, Microbiology, QR1-502 |
| More Details: | AIMP2-DX2, an exon 2-deleted splice variant of AIMP2 (aminoacyl-tRNA synthetase-interacting multifunctional protein 2), is highly expressed in lung cancer and involved in tumor progression in vivo. Oncogenic function of AIMP2-DX2 and its correlation with poor prognosis of cancer patients have been well established; however, the application of this potentially important biomarker to cancer research and diagnosis has been hampered by a lack of antibodies specific for the splice variant, possibly due to the poor immunogenicity and/or stability of AIMP2-DX2. In this study a monoclonal antibody, H5, that specifically recognizes AIMP2-DX2 and its isoforms was generated via rabbit immunization and phage display techniques, using a short peptide corresponding to the exon 1/3 junction sequence as an antigen. Furthermore, based on mutagenesis, limited cleavage, and mass spectrometry studies, it is also suggested that the endogenous isoform of AIMP2-DX2 recognized by H5 is produced by proteolytic cleavage of 33 amino acids from N-terminus and is capable of inducing cell proliferation similarly to the uncleaved protein. H5 monoclonal antibody is applicable to enzyme-linked immunosorbent assay, immunoblot, immunofluorescence, and immunohistochemistry, and expected to be a valuable tool for detecting AIMP2-DX2 with high sensitivity and specificity for research and diagnostic purposes. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 10060820 2218-273X |
| Relation: | https://www.mdpi.com/2218-273X/10/6/820; https://doaj.org/toc/2218-273X |
| DOI: | 10.3390/biom10060820 |
| Access URL: | https://doaj.org/article/f3bea71a026e4f46840e14efab5ddd6b |
| Accession Number: | edsdoj.f3bea71a026e4f46840e14efab5ddd6b |
| Database: | Directory of Open Access Journals |
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| ISSN: | 10060820 2218273X |
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| DOI: | 10.3390/biom10060820 |
| Published in: | Biomolecules |
| Language: | English |