Karyotype and genome size of Iberochondrostoma almacai (Teleostei, Cyprinidae) and comparison with the sister-species I. lusitanicum
| Title: | Karyotype and genome size of Iberochondrostoma almacai (Teleostei, Cyprinidae) and comparison with the sister-species I. lusitanicum |
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| Authors: | Rita Monteiro, Cláudia Carvalho, Maria João Collares-Pereira |
| Source: | Genetics and Molecular Biology, Vol 32, Iss 2, Pp 268-275 (2009) |
| Publisher Information: | Sociedade Brasileira de Genética, 2009. |
| Publication Year: | 2009 |
| Collection: | LCC:Genetics |
| Subject Terms: | Ag/CMA3-banding, flow cytometry, NORs, chromosome polymorphism, spontaneous triploidy, Genetics, QH426-470 |
| More Details: | This study aimed to define the karyotype of the recently described Iberian endemic Iberochondrostoma almacai, to revisit the previously documented chromosome polymorphisms of its sister species I. lusitanicum using C-, Ag-/CMA3 and RE-banding, and to compare the two species genome sizes. A 2n = 50 karyotype (with the exception of a triploid I. lusitanicum specimen) and a corresponding haploid chromosome formula of 7M:15SM:3A (FN = 94) were found. Multiple NORs were observed in both species (in two submetacentric chromosome pairs, one of them clearly homologous) and a higher intra and interpopulational variability was evidenced in I. lusitanicum. Flow cytometry measurements of nuclear DNA content showed some significant differences in genome size both between and within species: the genome of I. almacai was smaller than that of I. lusitanicum (mean values 2.61 and 2.93 pg, respectively), which presented a clear interpopulational variability (mean values ranging from 2.72 to 3.00 pg). These data allowed the distinction of both taxa and confirmed the existence of two well differentiated groups within I. lusitanicum: one that includes the populations from the right bank of the Tejo and Samarra drainages, and another that reunites the southern populations. The peculiar differences between the two species, presently listed as "Critically Endangered", reinforced the importance of this study for future conservation plans. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1415-4757 1678-4685 |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200011; https://doaj.org/toc/1415-4757; https://doaj.org/toc/1678-4685 |
| DOI: | 10.1590/S1415-47572009000200011 |
| Access URL: | https://doaj.org/article/f301a881c7b34e45a2215d5918695b68 |
| Accession Number: | edsdoj.f301a881c7b34e45a2215d5918695b68 |
| Database: | Directory of Open Access Journals |
| ISSN: | 14154757 16784685 |
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| DOI: | 10.1590/S1415-47572009000200011 |
| Published in: | Genetics and Molecular Biology |
| Language: | English |