Ursolic acid induces apoptosis and autophagy of HCT-8/5-FU cells

Bibliographic Details
Title:	Ursolic acid induces apoptosis and autophagy of HCT-8/5-FU cells
Authors:	Jinyan ZHAO, Shilan CHEN, Xuejiao WANG, Haixia SHANG, Jiao PENG, Jiumao LIN
Source:	Journal of Holistic Integrative Pharmacy, Vol 3, Iss 4, Pp 315-323 (2022)
Publisher Information:	KeAi Communications Co., Ltd., 2022.
Publication Year:	2022
Collection:	LCC:Pharmacy and materia medica LCC:Therapeutics. Pharmacology
Subject Terms:	Ursolic acid, Colorectal cancer, Multidrug resistance, Apoptosis, Autophagy, Mammalian target of rapamycin (mTOR), Pharmacy and materia medica, RS1-441, Therapeutics. Pharmacology, RM1-950
More Details:	[Abstract]: Objective: To observe the effects of ursolic acid (UA) on the apoptosis, autophagy and mTOR of human colorectal cancer (CRC) cells resistant to 5-FU (HCT-8/5-FU), and explore the mechanisms of UA reversing the multidrug resistance (MDR) of CRC. Methods: HCT-8 cells and its resistant cells to 5-FU (HCT-8/5-FU) were cultured to calculate the resistant index and confirmed the resistance of HCT-8/5-FU. The MTT assays were used to screen the suitable concentrations and the reversal fold of UA. The efflux function, proliferation, apoptosis, autophagy and their correlated proteins were determined through Doxorubucin staining, Rhodamine 123 staining, colony formation, Annexin V-PI double staining, Cyto-ID staining and Western blot after the HCT-8/5-FU were intervened with UA for 48 h. Results: HCT-8/5-FU cells are resistant to different chemotherapeutics, UA could reverse the MDR effectively. Furtherly, UA down-regulates the expression of ABCB1, ABCG2, p62, up-regulates Bax/Bcl-2 and LC3II/LC3I to inhibit the drug-efflux (P
Document Type:	article
File Description:	electronic resource
Language:	English
ISSN:	2707-3688
Relation:	http://www.sciencedirect.com/science/article/pii/S2707368823000018; https://doaj.org/toc/2707-3688
DOI:	10.1016/S2707-3688(23)00001-8
Access URL:	https://doaj.org/article/f274f9f2c43f45e19c1b20c8c04b7429
Accession Number:	edsdoj.f274f9f2c43f45e19c1b20c8c04b7429
Database:	Directory of Open Access Journals

More Details
ISSN:	27073688
DOI:	10.1016/S2707-3688(23)00001-8
Published in:	Journal of Holistic Integrative Pharmacy
Language:	English