Markers of viral hepatitis E (Hepeviridae, Orthohepevirus, Orthohepevirus A) in the imported Old World monkeys
| Title: | Markers of viral hepatitis E (Hepeviridae, Orthohepevirus, Orthohepevirus A) in the imported Old World monkeys |
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| Authors: | D. I. Dogadov, L. I. Korzaya, K. K. Kyuregyan, A. A. Karlsen, M. I. Mikhailov |
| Source: | Вопросы вирусологии, Vol 66, Iss 3, Pp 182-188 (2021) |
| Publisher Information: | Central Research Institute for Epidemiology, 2021. |
| Publication Year: | 2021 |
| Collection: | LCC:Microbiology |
| Subject Terms: | hepatitis e virus (hev), monkey, anti-hev (igg, igm), hev rna, Microbiology, QR1-502 |
| More Details: | Introduction. Viral hepatitis E is a zooanthroponotic disease that occurs in humans and various animals, including monkeys. It is caused by hepatitis E virus (HEV) (Hepeviridae, Orthohepevirus: Orthohepevirus A), for which 8 genotypes have been described to date. Among them, strains of genotypes 1 and 2 have been isolated from humans, strains of genotypes 3 and 4 from humans and animals, and strains of genotypes 5–8 from animals only. The main threat of the disease is associated with the documented zoonotic transmission of HEV genotypes 3, 4, 7, and 8, to humans through infected meat, blood and milk. Thus, monkeys could be involved in the transmission of HEV. The aim of this work was to study serological and molecular genetic markers of HEV infection in strepsirrhines (Old World monkeys, Cercopithecoidea), imported to the Adler Primate Center from various regions of the world (Tanzania, Vietnam, Mauritius). Material and methods. Fecal (n = 224) and blood serum samples (n = 395) from cynomolgus (Macaca fascicularis) and vervet monkeys (Chlorocebus pygerythrus) were examined by the enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR). Results and discussion. The data obtained show the high detection rate (51.8%) of IgG antibodies to HEV among 5 groups of cynomolgus monkeys imported from Vietnam, with a predominance of highly reactive sera (84%). High detection rate of IgM antibodies in these animals (10.4%) was observed, with the large number of IgM-reactive sera in one particular group of animals (36.8%). The fact of detection of HEV RNA in two groups of cynomolgus monkeys (11.9% and 5.7%) is of particular importance. All HEV sequences of isolated from monkeys belonged to genotype 4. Conclusion. Our data indicate that monkeys (in particular, cynomolgus monkeys) can serve as a natural reservoir of HEV genotype 4 for humans. This requires an appropriate set of anti-epidemic measures in a number of situations. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English Russian |
| ISSN: | 0507-4088 2411-2097 |
| Relation: | https://virusjour.crie.ru/jour/article/viewFile/520/319; https://doaj.org/toc/0507-4088; https://doaj.org/toc/2411-2097 |
| DOI: | 10.36233/0507-4088-34 |
| Access URL: | https://doaj.org/article/af24a5229e954679b5657b1e61170ae9 |
| Accession Number: | edsdoj.f24a5229e954679b5657b1e61170ae9 |
| Database: | Directory of Open Access Journals |
| ISSN: | 05074088 24112097 |
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| DOI: | 10.36233/0507-4088-34 |
| Published in: | Вопросы вирусологии |
| Language: | English Russian |