Academic Journal
Whole-genome transcriptome and DNA methylation dynamics of pre-implantation embryos reveal progression of embryonic genome activation in buffaloes
| Title: | Whole-genome transcriptome and DNA methylation dynamics of pre-implantation embryos reveal progression of embryonic genome activation in buffaloes |
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| Authors: | Penghui Fu, Du Zhang, Chunyan Yang, Xiang Yuan, Xier Luo, Haiying Zheng, Yanfei Deng, Qingyou Liu, Kuiqing Cui, Fei Gao, Deshun Shi |
| Source: | Journal of Animal Science and Biotechnology, Vol 14, Iss 1, Pp 1-17 (2023) |
| Publisher Information: | BMC, 2023. |
| Publication Year: | 2023 |
| Collection: | LCC:Animal culture LCC:Veterinary medicine |
| Subject Terms: | Buffalo, DNA methylome, Embryonic genome activation, Maternal-to-zygote transition, Transcriptome, Animal culture, SF1-1100, Veterinary medicine, SF600-1100 |
| More Details: | Abstract Background During mammalian pre-implantation embryonic development (PED), the process of maternal-to-zygote transition (MZT) is well orchestrated by epigenetic modification and gene sequential expression, and it is related to the embryonic genome activation (EGA). During MZT, the embryos are sensitive to the environment and easy to arrest at this stage in vitro. However, the timing and regulation mechanism of EGA in buffaloes remain obscure. Results Buffalo pre-implantation embryos were subjected to trace cell based RNA-seq and whole-genome bisulfite sequencing (WGBS) to draw landscapes of transcription and DNA-methylation. Four typical developmental steps were classified during buffalo PED. Buffalo major EGA was identified at the 16-cell stage by the comprehensive analysis of gene expression and DNA methylation dynamics. By weighted gene co-expression network analysis, stage-specific modules were identified during buffalo maternal-to-zygotic transition, and key signaling pathways and biological process events were further revealed. Programmed and continuous activation of these pathways was necessary for success of buffalo EGA. In addition, the hub gene, CDK1, was identified to play a critical role in buffalo EGA. Conclusions Our study provides a landscape of transcription and DNA methylation in buffalo PED and reveals deeply the molecular mechanism of the buffalo EGA and genetic programming during buffalo MZT. It will lay a foundation for improving the in vitro development of buffalo embryos. Graphical Abstract |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2049-1891 |
| Relation: | https://doaj.org/toc/2049-1891 |
| DOI: | 10.1186/s40104-023-00894-5 |
| Access URL: | https://doaj.org/article/f2223eb915484b158d60702a5c961b01 |
| Accession Number: | edsdoj.f2223eb915484b158d60702a5c961b01 |
| Database: | Directory of Open Access Journals |
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| ISSN: | 20491891 |
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| DOI: | 10.1186/s40104-023-00894-5 |
| Published in: | Journal of Animal Science and Biotechnology |
| Language: | English |