Performance of direct immunofluorescence assay for the detection of human metapneumovirus under clinical laboratory settings
| Title: | Performance of direct immunofluorescence assay for the detection of human metapneumovirus under clinical laboratory settings |
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| Authors: | Jonas Michel Wolf, Tatiana Schäffer Gregianini, Claudete Maria Farina Seadi, Gabriela Luchiari Tumioto, Bibiana Paula Dambrós, Fernanda Kieling Moreira Lehmann, Silvia De Carli, Nilo Ikuta, Vagner Ricardo Lunge |
| Source: | Revista da Sociedade Brasileira de Medicina Tropical, Vol 48, Iss 6, Pp 762-764 (2015) |
| Publisher Information: | Sociedade Brasileira de Medicina Tropical (SBMT), 2015. |
| Publication Year: | 2015 |
| Collection: | LCC:Arctic medicine. Tropical medicine |
| Subject Terms: | Human metapneumovirus, Direct immunofluorescence, qRT-PCR, Arctic medicine. Tropical medicine, RC955-962 |
| More Details: | ABSTRACT INTRODUCTION: Human metapneumovirus (hMPV) is an emergent human respiratory pathogen. This study aimed to evaluate the performance of direct immunofluorescence (DIF) to detect hMPV in a clinical laboratory setting. METHODS: Nasopharyngeal aspirate samples (448) of children and adults with respiratory illness were used to detect hMPV by using DIF and real time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays. RESULTS: In all, 36 (8%) samples were positive by DIF and 94 (21%) were positive by qRT-PCR. Direct immunofluorescence specificity was 99% and sensitivity was 38%. CONCLUSIONS: DIF is not very sensitive under clinical laboratory settings. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1678-9849 0037-8682 |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000600762&lng=en&tlng=en; https://doaj.org/toc/1678-9849 |
| DOI: | 10.1590/0037-8682-0107-2015 |
| Access URL: | https://doaj.org/article/af20c246748d4a939ba1273474ac932e |
| Accession Number: | edsdoj.f20c246748d4a939ba1273474ac932e |
| Database: | Directory of Open Access Journals |
| ISSN: | 16789849 00378682 |
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| DOI: | 10.1590/0037-8682-0107-2015 |
| Published in: | Revista da Sociedade Brasileira de Medicina Tropical |
| Language: | English |