Bibliographic Details
| Title: |
The Isolation and Structure Elucidation of Spirotetronate Lobophorins A, B, and H8 from Streptomyces sp. CB09030 and Their Biosynthetic Gene Cluster |
| Authors: |
Jie Shi, Dian Peng, Fei-Fei Peng, Qing-Bo Zhang, Yan-Wen Duan, Yong Huang |
| Source: |
Molecules, Vol 28, Iss 8, p 3597 (2023) |
| Publisher Information: |
MDPI AG, 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Organic chemistry |
| Subject Terms: |
Streptomyces, spirotetronate, lobophorins, transwell, anti-mycobacterial activity, Organic chemistry, QD241-441 |
| More Details: |
Lobophorins (LOBs) are a growing family of spirotetronate natural products with significant cytotoxicity, anti-inflammatory, and antibacterial activities. Herein, we report the transwell-based discovery of Streptomyces sp. CB09030 from a panel of 16 in-house Streptomyces strains, which has significant anti-mycobacterial activity and produces LOB A (1), LOB B (2), and LOB H8 (3). Genome sequencing and bioinformatic analyses revealed the potential biosynthetic gene cluster (BGC) for 1–3, which is highly homologous with the reported BGCs for LOBs. However, the glycosyltransferase LobG1 in S. sp. CB09030 has certain point mutations compared to the reported LobG1. Finally, LOB analogue 4 (O-β-D-kijanosyl-(1→17)-kijanolide) was obtained through an acid-catalyzed hydrolysis of 2. Compounds 1–4 showed different antibacterial activities against Mycobacterium smegmatis and Bacillus subtilis, which revealed the varying roles of different sugars in their antibacterial activities. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1420-3049 |
| Relation: |
https://www.mdpi.com/1420-3049/28/8/3597; https://doaj.org/toc/1420-3049 |
| DOI: |
10.3390/molecules28083597 |
| Access URL: |
https://doaj.org/article/f16f43b4388b48dd8685333bc6d52701 |
| Accession Number: |
edsdoj.f16f43b4388b48dd8685333bc6d52701 |
| Database: |
Directory of Open Access Journals |
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