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Apoptosis and autophagy induction of Seleno-β-lactoglobulin (Se-β-Lg) on hepatocellular carcinoma cells lines

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Title: Apoptosis and autophagy induction of Seleno-β-lactoglobulin (Se-β-Lg) on hepatocellular carcinoma cells lines
Authors: Yana Zhao, Yaowei Liu, Wenhang Wang, Di Wu, Jianhui Shi, Anjun Liu
Source: Journal of Functional Foods, Vol 49, Iss , Pp 412-423 (2018)
Publisher Information: Elsevier, 2018.
Publication Year: 2018
Collection: LCC:Nutrition. Foods and food supply
Subject Terms: Seleno-β-lactoglobulin, Hepatocellular carcinoma cells lines, Autophagy, Apoptosis pathway, p53, Nutrition. Foods and food supply, TX341-641
More Details: β-lactoglobulin is important fraction in bovine milk. Selenium is an essential nutrient element. The apoptosis and autophagy induction of Se-β-Lg combined selenic acid in β-lactoglobulin on Hep G2 and Hep 3B cells were investigated in this study. MTT assay showed Se-β-Lg inhibited cells viability. Annexin V-FITC/PI and PI staining indicated Se-β-Lg triggered cells apoptosis and cell cycle arrest. Western blot of caspase-cascade suggested Hep G2 and Hep 3B cells occurred irreversible extrinsic apoptosis. TEM and protein expression of LC3 indicated Se-β-Lg induced cells autophagy. Further explore found Se-β-Lg induced up-regulation of p53 and Fas/FasL in Hep G2 cells. Simultaneously, N-acetyl-L-cysteine, pifithrin-α, Z-VAD-FMK and 3-MA were used. These results further demonstrated Se-β-Lg triggered autophagy and receptor-related apoptosis via p53-mediated Fas/FasL pathway in Hep G2 cells and induced autophagy and p53/Fas-independent caspase activation in Hep 3B cells. Our research revealed that Se-β-Lg had potential to act as chemopreventive compounds in HCC therapy.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 1756-4646
Relation: http://www.sciencedirect.com/science/article/pii/S1756464618304882; https://doaj.org/toc/1756-4646
DOI: 10.1016/j.jff.2018.09.011
Access URL: https://doaj.org/article/f08bcfae931842438d6b7b6462ffa0c6
Accession Number: edsdoj.f08bcfae931842438d6b7b6462ffa0c6
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  Data: Apoptosis and autophagy induction of Seleno-β-lactoglobulin (Se-β-Lg) on hepatocellular carcinoma cells lines
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  Data: <searchLink fieldCode="AR" term="%22Yana+Zhao%22">Yana Zhao</searchLink><br /><searchLink fieldCode="AR" term="%22Yaowei+Liu%22">Yaowei Liu</searchLink><br /><searchLink fieldCode="AR" term="%22Wenhang+Wang%22">Wenhang Wang</searchLink><br /><searchLink fieldCode="AR" term="%22Di+Wu%22">Di Wu</searchLink><br /><searchLink fieldCode="AR" term="%22Jianhui+Shi%22">Jianhui Shi</searchLink><br /><searchLink fieldCode="AR" term="%22Anjun+Liu%22">Anjun Liu</searchLink>
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  Data: Journal of Functional Foods, Vol 49, Iss , Pp 412-423 (2018)
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  Data: <searchLink fieldCode="DE" term="%22Seleno-β-lactoglobulin%22">Seleno-β-lactoglobulin</searchLink><br /><searchLink fieldCode="DE" term="%22Hepatocellular+carcinoma+cells+lines%22">Hepatocellular carcinoma cells lines</searchLink><br /><searchLink fieldCode="DE" term="%22Autophagy%22">Autophagy</searchLink><br /><searchLink fieldCode="DE" term="%22Apoptosis+pathway%22">Apoptosis pathway</searchLink><br /><searchLink fieldCode="DE" term="%22p53%22">p53</searchLink><br /><searchLink fieldCode="DE" term="%22Nutrition%2E+Foods+and+food+supply%22">Nutrition. Foods and food supply</searchLink><br /><searchLink fieldCode="DE" term="%22TX341-641%22">TX341-641</searchLink>
– Name: Abstract
  Label: Description
  Group: Ab
  Data: β-lactoglobulin is important fraction in bovine milk. Selenium is an essential nutrient element. The apoptosis and autophagy induction of Se-β-Lg combined selenic acid in β-lactoglobulin on Hep G2 and Hep 3B cells were investigated in this study. MTT assay showed Se-β-Lg inhibited cells viability. Annexin V-FITC/PI and PI staining indicated Se-β-Lg triggered cells apoptosis and cell cycle arrest. Western blot of caspase-cascade suggested Hep G2 and Hep 3B cells occurred irreversible extrinsic apoptosis. TEM and protein expression of LC3 indicated Se-β-Lg induced cells autophagy. Further explore found Se-β-Lg induced up-regulation of p53 and Fas/FasL in Hep G2 cells. Simultaneously, N-acetyl-L-cysteine, pifithrin-α, Z-VAD-FMK and 3-MA were used. These results further demonstrated Se-β-Lg triggered autophagy and receptor-related apoptosis via p53-mediated Fas/FasL pathway in Hep G2 cells and induced autophagy and p53/Fas-independent caspase activation in Hep 3B cells. Our research revealed that Se-β-Lg had potential to act as chemopreventive compounds in HCC therapy.
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        Value: 10.1016/j.jff.2018.09.011
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      – Text: English
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    Subjects:
      – SubjectFull: Seleno-β-lactoglobulin
        Type: general
      – SubjectFull: Hepatocellular carcinoma cells lines
        Type: general
      – SubjectFull: Autophagy
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      – SubjectFull: TX341-641
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      – TitleFull: Apoptosis and autophagy induction of Seleno-β-lactoglobulin (Se-β-Lg) on hepatocellular carcinoma cells lines
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            NameFull: Yana Zhao
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