Bibliographic Details
| Title: |
Apoptosis and autophagy induction of Seleno-β-lactoglobulin (Se-β-Lg) on hepatocellular carcinoma cells lines |
| Authors: |
Yana Zhao, Yaowei Liu, Wenhang Wang, Di Wu, Jianhui Shi, Anjun Liu |
| Source: |
Journal of Functional Foods, Vol 49, Iss , Pp 412-423 (2018) |
| Publisher Information: |
Elsevier, 2018. |
| Publication Year: |
2018 |
| Collection: |
LCC:Nutrition. Foods and food supply |
| Subject Terms: |
Seleno-β-lactoglobulin, Hepatocellular carcinoma cells lines, Autophagy, Apoptosis pathway, p53, Nutrition. Foods and food supply, TX341-641 |
| More Details: |
β-lactoglobulin is important fraction in bovine milk. Selenium is an essential nutrient element. The apoptosis and autophagy induction of Se-β-Lg combined selenic acid in β-lactoglobulin on Hep G2 and Hep 3B cells were investigated in this study. MTT assay showed Se-β-Lg inhibited cells viability. Annexin V-FITC/PI and PI staining indicated Se-β-Lg triggered cells apoptosis and cell cycle arrest. Western blot of caspase-cascade suggested Hep G2 and Hep 3B cells occurred irreversible extrinsic apoptosis. TEM and protein expression of LC3 indicated Se-β-Lg induced cells autophagy. Further explore found Se-β-Lg induced up-regulation of p53 and Fas/FasL in Hep G2 cells. Simultaneously, N-acetyl-L-cysteine, pifithrin-α, Z-VAD-FMK and 3-MA were used. These results further demonstrated Se-β-Lg triggered autophagy and receptor-related apoptosis via p53-mediated Fas/FasL pathway in Hep G2 cells and induced autophagy and p53/Fas-independent caspase activation in Hep 3B cells. Our research revealed that Se-β-Lg had potential to act as chemopreventive compounds in HCC therapy. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1756-4646 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S1756464618304882; https://doaj.org/toc/1756-4646 |
| DOI: |
10.1016/j.jff.2018.09.011 |
| Access URL: |
https://doaj.org/article/f08bcfae931842438d6b7b6462ffa0c6 |
| Accession Number: |
edsdoj.f08bcfae931842438d6b7b6462ffa0c6 |
| Database: |
Directory of Open Access Journals |
