Bibliographic Details
| Title: |
PhoP: a missing piece in the intricate puzzle of Mycobacterium tuberculosis virulence. |
| Authors: |
Jesús Gonzalo-Asensio, Serge Mostowy, Jose Harders-Westerveen, Kris Huygen, Rogelio Hernández-Pando, Jelle Thole, Marcel Behr, Brigitte Gicquel, Carlos Martín |
| Source: |
PLoS ONE, Vol 3, Iss 10, p e3496 (2008) |
| Publisher Information: |
Public Library of Science (PLoS), 2008. |
| Publication Year: |
2008 |
| Collection: |
LCC:Medicine
LCC:Science |
| Subject Terms: |
Medicine, Science |
| More Details: |
Inactivation of the transcriptional regulator PhoP results in Mycobacterium tuberculosis attenuation. Preclinical testing has shown that attenuated M. tuberculosis phoP mutants hold promise as safe and effective live vaccine candidates. We focused this study to decipher the virulence networks regulated by PhoP. A combined transcriptomic and proteomic analysis revealed that PhoP controls a variety of functions including: hypoxia response through DosR crosstalking, respiratory metabolism, secretion of the major T-cell antigen ESAT-6, stress response, synthesis of pathogenic lipids and the M. tuberculosis persistence through transcriptional regulation of the enzyme isocitrate lyase. We also demonstrate that the M. tuberculosis phoP mutant SO2 exhibits an antigenic capacity similar to that of the BCG vaccine. Finally, we provide evidence that the SO2 mutant persists better in mouse organs than BCG. Altogether, these findings indicate that PhoP orchestrates a variety of functions implicated in M. tuberculosis virulence and persistence, making phoP mutants promising vaccine candidates. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1932-6203 |
| Relation: |
http://europepmc.org/articles/PMC2566814?pdf=render; https://doaj.org/toc/1932-6203 |
| DOI: |
10.1371/journal.pone.0003496 |
| Access URL: |
https://doaj.org/article/acf078ffa7584179b0b1223916c2ab17 |
| Accession Number: |
edsdoj.f078ffa7584179b0b1223916c2ab17 |
| Database: |
Directory of Open Access Journals |
