Proteomics Identifies LUC7L3 as a Prognostic Biomarker for Hepatocellular Carcinoma
| Title: | Proteomics Identifies LUC7L3 as a Prognostic Biomarker for Hepatocellular Carcinoma |
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| Authors: | Yushan Hou, Siqi Wang, Yiming Zhang, Xiaofen Huang, Xiuyuan Zhang, Fuchu He, Chunyan Tian, Aihua Sun |
| Source: | Current Issues in Molecular Biology, Vol 46, Iss 5, Pp 4004-4020 (2024) |
| Publisher Information: | MDPI AG, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Biology (General) |
| Subject Terms: | hepatocellular carcinoma, alternative splicing, LUC7L3, RRM2, prognostic biomarker, Biology (General), QH301-705.5 |
| More Details: | Alternative splicing has been shown to participate in tumor progression, including hepatocellular carcinoma. The poor prognosis of patients with HCC calls for molecular classification and biomarker identification to facilitate precision medicine. We performed ssGSEA analysis to quantify the pathway activity of RNA splicing in three HCC cohorts. Kaplan–Meier and Cox methods were used for survival analysis. GO and GSEA were performed to analyze pathway enrichment. We confirmed that RNA splicing is significantly correlated with prognosis, and identified an alternative splicing-associated protein LUC7L3 as a potential HCC prognostic biomarker. Further bioinformatics analysis revealed that high LUC7L3 expression indicated a more progressive HCC subtype and worse clinical features. Cell proliferation-related pathways were enriched in HCC patients with high LUC7L3 expression. Consistently, we proved that LUC7L3 knockdown could significantly inhibit cell proliferation and suppress the activation of associated signaling pathways in vitro. In this research, the relevance between RNA splicing and HCC patient prognosis was outlined. Our newly identified biomarker LUC7L3 could provide stratification for patient survival and recurrence risk, facilitating early medical intervention before recurrence or disease progression. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1467-3045 1467-3037 |
| Relation: | https://www.mdpi.com/1467-3045/46/5/247; https://doaj.org/toc/1467-3037; https://doaj.org/toc/1467-3045 |
| DOI: | 10.3390/cimb46050247 |
| Access URL: | https://doaj.org/article/ebfd8a674e9f468480b9e7f0d5982f64 |
| Accession Number: | edsdoj.bfd8a674e9f468480b9e7f0d5982f64 |
| Database: | Directory of Open Access Journals |
| ISSN: | 14673045 14673037 |
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| DOI: | 10.3390/cimb46050247 |
| Published in: | Current Issues in Molecular Biology |
| Language: | English |