Academic Journal
Proteome Profiling of Cucurbita pepo Phyllosphere After Infection by Podosphaera xanthii and Application of Reynoutria sachalinensis Extract
| Title: | Proteome Profiling of Cucurbita pepo Phyllosphere After Infection by Podosphaera xanthii and Application of Reynoutria sachalinensis Extract |
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| Authors: | Ioannis Theologidis, Manousos Makridakis, Aikaterini Termentzi, Eirini Baira, Jerome Zoidakis, Dimosthenis Kizis |
| Source: | Applied Sciences, Vol 14, Iss 21, p 10061 (2024) |
| Publisher Information: | MDPI AG, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Technology LCC:Engineering (General). Civil engineering (General) LCC:Biology (General) LCC:Physics LCC:Chemistry |
| Subject Terms: | Cucurbita pepo, zucchini, Podosphaera xanthii, powdery mildew, Reynoutria sachalinensis, elicitors, Technology, Engineering (General). Civil engineering (General), TA1-2040, Biology (General), QH301-705.5, Physics, QC1-999, Chemistry, QD1-999 |
| More Details: | Podosphaera xanthii is the main causal agent of powdery mildew (PM) disease for Cucurbita pepo. Disease control is attained principally by applications of chemical fungicides, along with parallel use of tolerant crop varieties and alternate application of elicitors to control development of disease resistance. To get insight into C. pepo molecular responses to P. xanthii infection and elicitor treatment we studied the proteomic profile differences at the phyllosphere of a zucchini cultivar susceptible to PM, at the onset of P. xanthii (PX) infection and after application of Reynoutria sachalinensis (RS) plant extract, respectively, using a nano-LC-HRMS/MS, Q-Exactive-Orbitrap approach. Analysis of peptide sequences regarding four treatment groups (Control; PX; RS; and RSPX (PX-infected priorly treated with RS)) resulted in 2070 CuGenDB annotations. Three comparisons (treatments vs. Control) encompassed most of the Differentially Expressed Proteins (DEPs). In these three comparisons, KEGG and Gene Ontology functional analyses highlighted unique differentially enriched pathways—some of which included highly expressed proteins—in PX-related (proteasome, pentose phosphate pathway, and carbon fixation), RS-related (biosynthesis of secondary metabolites, flavonoids, and starch and sucrose metabolism), and RSPX-related (pyruvate metabolism and polycomb repressive complex) comparisons, respectively, suggesting distinct mechanisms of early plant responses modulated by PX and RS. Furthermore, in four out of six comparisons the thiamine metabolism pathway was found to be enriched, suggesting a pivotal role in PX-induced responses. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2076-3417 |
| Relation: | https://www.mdpi.com/2076-3417/14/21/10061; https://doaj.org/toc/2076-3417 |
| DOI: | 10.3390/app142110061 |
| Access URL: | https://doaj.org/article/bee93ecd7b95472ba88d35476b323630 |
| Accession Number: | edsdoj.bee93ecd7b95472ba88d35476b323630 |
| Database: | Directory of Open Access Journals |
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| ISSN: | 20763417 |
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| DOI: | 10.3390/app142110061 |
| Published in: | Applied Sciences |
| Language: | English |